EP 0975969 A4 20020731 - METHODS FOR SCREENING COMPOUNDS USING ENCAPSULATED CELLS
Title (en)
METHODS FOR SCREENING COMPOUNDS USING ENCAPSULATED CELLS
Title (de)
SCREENING-METHODE FÜR VERBINDUNGEN MIT HILFE EINGEKAPSELTER ZELLEN
Title (fr)
METHODES DE TRI DE COMPOSES AU MOYEN DE CELLULES ENCAPSULEES
Publication
Application
Priority
- US 9805462 W 19980318
- US 4088897 P 19970318
- US 7104698 P 19980113
Abstract (en)
[origin: WO9841869A1] The present invention relates to an integrated approach to drug screening that is designed to couple a screening assay both temporally and spatially to natural product synthesis in a microorganism. The present invention provides a screening unit which is a gel droplet comprising a producing species that produce natural products for the drug screen, and an assay system that detects or measures a desired biological activity. A producing species is coencapsulated with an assay system in a screening unit when the producing species is at a phase in its life cycle that is optimal for producing natural products, such as secondary metabolites. The producing species is spatially positioned relative to the assay system in the same unit such that compounds produced by the producing species can come into contact with the assay system. If a compound possesses the desired activity, the assay system will generate a signal that enables the identification and/or isolation of the screening unit. The present invention also provides methods for forming a screening unit, and methods for using a screening unit in drug screening. The methods are useful for screening naturally occurring organisms as well as genetically engineered cells, and solid carriers containing chemicals.
IPC 1-7
IPC 8 full level
G01N 33/50 (2006.01)
CPC (source: EP)
G01N 33/5008 (2013.01); G01N 33/502 (2013.01); G01N 2333/36 (2013.01)
Citation (search report)
- [X] GB 2159171 A 19851127 - DAMON BIOTECH INC
- [XY] WO 8910786 A2 19891116 - MICRODROP INC [US]
- [X] WO 9428119 A1 19941208 - SMITHKLINE BEECHAM PLC [GB], et al
- [YD] WO 9634112 A1 19961031 - CHROMAXOME CORP [US]
- [X] RYAN C. ET AL.: "Rapid assay for mycobacterial growth and antibiotic susceptibility using gel microdrop encapsulation.", J. CLIN. MICROBIOL., vol. 33, no. 7, July 1995 (1995-07-01), pages 1720 - 1726, XP002190454
- [YD] MC DANIEL R. ET AL.: "Engineered biosynthesis of novel polyketides.", SCIENCE, vol. 262, 3 December 1993 (1993-12-03), pages 1546 - 1550, XP000910221
- See references of WO 9841869A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9841869 A1 19980924; AU 6571598 A 19981012; CA 2284066 A1 19980924; EP 0975969 A1 20000202; EP 0975969 A4 20020731
DOCDB simple family (application)
US 9805462 W 19980318; AU 6571598 A 19980318; CA 2284066 A 19980318; EP 98911863 A 19980318