EP 0977885 A1 20000209 - METHODS FOR IDENTIFYING, CHARACTERIZING, AND EVOLVING CELL-TYPE SPECIFIC CIS REGULATORY ELEMENTS
Title (en)
METHODS FOR IDENTIFYING, CHARACTERIZING, AND EVOLVING CELL-TYPE SPECIFIC CIS REGULATORY ELEMENTS
Title (de)
VERFAHREN FÜR DIE IDENTIFIZIERUNG, CHARACTERISIERUNG UND VORTBILDUNG VON ZELL-TYPEN SPEZIFISCHE CIS REGULATORISCHE ELEMENTE
Title (fr)
PROCEDES POUR IDENTIFIER, CARACTERISER ET FAIRE EVOLUER DES ELEMENTS REGULATEURS A EFFET CIS SPECIFIQUE DE TYPE CELLULAIRE
Publication
Application
Priority
- US 9802758 W 19980213
- US 80066497 A 19970214
Abstract (en)
[origin: WO9836097A1] The invention provides methods for efficient and rapid identification of cis-acting nucleic acid sequences that act in a cell-type specific manner to stimulate or repress the expression of linked genes or other neighboring sequences. The invention also provides methods for evolving novel regulatory sequences by in vitro manipulation of naturally occurring or synthetic cis-acting nucleic acid sequences followed by screening and counterscreening steps. Furthermore, the invention provides methods for determining the mechanism by which cell-type specific cis regulatory sequences confer cell-type specific expression. Also provided are diagnostic methods based on the use of cell-type specific cis regulatory sequences.
IPC 1-7
IPC 8 full level
C12N 15/10 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP US)
C12N 15/1051 (2013.01 - EP US); C12Q 1/6897 (2013.01 - EP US)
Citation (search report)
See references of WO 9836097A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9836097 A1 19980820; AU 6161698 A 19980908; CA 2281016 A1 19980820; EP 0977885 A1 20000209; US 6623922 B1 20030923
DOCDB simple family (application)
US 9802758 W 19980213; AU 6161698 A 19980213; CA 2281016 A 19980213; EP 98906378 A 19980213; US 80066497 A 19970214