EP 0977994 A1 20000209 - METHOD FOR THE IDENTIFICATION AND CHARACTERIZATION OF NUCLEAR RECEPTOR LIGANDS
Title (en)
METHOD FOR THE IDENTIFICATION AND CHARACTERIZATION OF NUCLEAR RECEPTOR LIGANDS
Title (de)
METHODE ZUR IDENTIFIZIERUNG UND CHARAKTERISIERUNG VON LIGANDEN NUKLEÄRER REZEPTOREN
Title (fr)
METHODE D'IDENTIFICATION ET DE CARACTERISATION DES LIGANDS DES RECEPTEURS NUCLEAIRES
Publication
Application
Priority
- US 9806672 W 19980406
- US 84556797 A 19970424
Abstract (en)
[origin: WO9848281A1] The present invention is directed to a methodology to study the effect of ligands that can modulate functions of nuclear receptors, and specifically, their interaction with specific DNA or other transcription factors. These receptors play a major role in the cascade of inter and intracellular physiological and biochemical events. They generally have a modular construction which enables them to recognize and bind to certain DNA sequences, or binding elements, such as hormone response elements. Various ligands, such as hormones, affect this interaction. In accordance with the methodology presented herein, measurements of such interactions are taken in real time and kinetic pattern. This allows the user to identify and to characterize ligands that are able to affect a receptor's interaction with its binding element. Hence, the present invention provides a useful tool for the elucidation, and development of ligands that affect transcription of target genes, either directly or indirectly.
IPC 1-7
IPC 8 full level
C12M 1/00 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); G01N 33/53 (2006.01); G01N 33/543 (2006.01); G01N 33/566 (2006.01); G01N 33/74 (2006.01)
CPC (source: EP)
G01N 33/743 (2013.01)
Citation (search report)
See references of WO 9848281A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9848281 A1 19981029; AU 6883198 A 19981113; CA 2286761 A1 19981029; EP 0977994 A1 20000209; JP 2002507276 A 20020305
DOCDB simple family (application)
US 9806672 W 19980406; AU 6883198 A 19980406; CA 2286761 A 19980406; EP 98914486 A 19980406; JP 54607398 A 19980406