EP 0979307 A2 20000216 - POLYNUCLEOTIDE SEQUENCING USING SEMI-DEGENERATE PRIMERS
Title (en)
POLYNUCLEOTIDE SEQUENCING USING SEMI-DEGENERATE PRIMERS
Title (de)
POLYNUKLEOTID SEQUENZIERUNG MIT HALB-DEGENERIERTEN PRIMERN
Title (fr)
SEQUEN AGE D'UN POLYNUCLEOTIDE AU MOYEN D'AMORCES SEMIDEGENEREES
Publication
Application
Priority
- GB 9801233 W 19980428
- GB 9708606 A 19970428
Abstract (en)
[origin: WO9849341A2] A method for the analysis of polynucleotide molecules comprises the steps of: a) reacting a target polynucleotide with oligonucleotide primers, a polymerase enzyme, and the other reagents necessary for the polymerase reaction, wherein the oligonucleotide primers are chosen such that polymerase products of varying lengths are produced; and b) analysing the products of the said reaction or reactions; wherein the oligonucleotide primers include an array of semi-degenerate primers whose (3') ends comprise variations of one or more nucleotides A, T, G, and C such that the array is complementary to all the polynucleotide sequence.
IPC 1-7
IPC 8 full level
C12Q 1/68 (2006.01); C12Q 1/6869 (2018.01)
CPC (source: EP)
C12Q 1/6869 (2013.01)
Citation (search report)
See references of WO 9849341A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9849341 A2 19981105; WO 9849341 A3 19990128; AU 7220398 A 19981124; EP 0979307 A2 20000216; GB 9708606 D0 19970618
DOCDB simple family (application)
GB 9801233 W 19980428; AU 7220398 A 19980428; EP 98919324 A 19980428; GB 9708606 A 19970428