EP 0980264 A2 20000223 - MAMMALIAN CELL TRANSDUCTION FOR USE IN GENE THERAPY
Title (en)
MAMMALIAN CELL TRANSDUCTION FOR USE IN GENE THERAPY
Title (de)
SÄUGETIERZELLTRANSDUKTION ZUR VERWENDUNG IN GENTHERAPIE
Title (fr)
TRANSDUCTION DE CELLULES DE MAMMIFERES UTILISEE EN THERAPIE GENIQUE
Publication
Application
Priority
- EP 98930718 A 19980518
- EP 9803013 W 19980518
- EP 97201480 A 19970516
- EP 98200382 A 19980209
Abstract (en)
[origin: WO9853063A2] The present invention relates to a method for the ex vivo transduction of mammalian cells, in particular to the transduction of bone marrow stromal cells. These cells can be transduced with a gene of interest, in particular a B-domain deleted human factor VIII gene. In the latter case, the transduced cells can be used to treat hemophilia A. The method for the ex vivo transduction of bone marrow stromal cells with the human factor VIII gene comprises provision of an intron-based retroviral vector comprising a B-domain deleted human factor VIII cDNA (designated as MFG-FVIII DELTA B); pseudotyping the said vector with the Gibbon ape leukemia virus (GALV) envelope; transducing bone marrow stromal cells with the said pseudotyped vector by pre-incubating the cells for a suitable period of time in cell culture medium without phosphate and subsequently adding a vector-containing supernatant, optionally supplemented with transduction additives to the cells, followed by centrifuging the mixture thus obtained; and optionally repeating the two previous steps.
IPC 1-7
IPC 8 full level
A61K 35/28 (2006.01); A61K 38/37 (2006.01); A61K 48/00 (2006.01); C12N 5/06 (2006.01); C12N 5/077 (2010.01); C12N 5/10 (2006.01); C12N 15/12 (2006.01); C12N 15/867 (2006.01)
CPC (source: EP)
A61K 38/37 (2013.01); C12N 5/0669 (2013.01); C12N 15/86 (2013.01); A61K 48/00 (2013.01); C12N 2740/13043 (2013.01); C12N 2840/203 (2013.01)
Citation (search report)
See references of WO 9853063A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9853063 A2 19981126; WO 9853063 A3 19990318; AU 8105798 A 19981211; CA 2289918 A1 19981126; EP 0980264 A2 20000223
DOCDB simple family (application)
EP 9803013 W 19980518; AU 8105798 A 19980518; CA 2289918 A 19980518; EP 98930718 A 19980518