EP 0980436 A1 20000223 - INCREASED PRODUCTION OF PROTEINS BY USING CHAPERONE-LIKE PROTEINS
Title (en)
INCREASED PRODUCTION OF PROTEINS BY USING CHAPERONE-LIKE PROTEINS
Title (de)
ERHÖHTE PRODUKTION VON PROTEINEN DURCH VERWENDUNG VON CHAPERONPROTEINEN
Title (fr)
PRODUCTION ACCRUE DE PROTEINES AU MOYEN DE PROTEINES DU TYPE CHAPERONE
Publication
Application
Priority
- EP 98928658 A 19980608
- EP 97201790 A 19970613
- NL 9800335 W 19980608
Abstract (en)
[origin: WO9856928A1] The invention relates to the production of recombinant proteins that are expressed in heterologous host cells and to methods to increase production levels of these proteins by the use of a chaperon-like protein. Producing or isolating a protein or polypeptide in large amounts and in a relatively pure form from its original (homologous) host or host cell where it is expressed is cumbersome and often economically unattractive. However, the development of recombinant DNA methodology has opened the possibility to produce proteins by expression, e.g. in a heterologous host or host cell. A wide and still expanding array of foreign polypeptides or proteins can now be produced or expressed in heterologous expression systems. The invention provides a method to express a polypeptide encoded by a nucleic acid sequence present in a host cell which host cell additionally comprises a chaperone-like protein capable of specific binding with said polypeptide.
IPC 1-7
C12N 15/62; C07K 19/00; C12P 21/02; C12N 15/70; C12N 15/74; C12N 15/81; C12N 15/80; C12N 1/21; C12N 1/15; C12N 1/16
IPC 8 full level
C07K 16/40 (2006.01); C07K 19/00 (2006.01); C12N 1/15 (2006.01); C12N 1/16 (2006.01); C12N 1/21 (2006.01); C12N 9/64 (2006.01); C12N 15/62 (2006.01); C12N 15/67 (2006.01); C12N 15/70 (2006.01); C12N 15/74 (2006.01); C12N 15/80 (2006.01); C12N 15/81 (2006.01); C12P 21/02 (2006.01)
CPC (source: EP)
C07K 16/40 (2013.01); C12N 9/6483 (2013.01); C12N 15/625 (2013.01); C12N 15/67 (2013.01); C12N 15/81 (2013.01); C12P 21/02 (2013.01); C12Y 304/23004 (2013.01); C07K 2319/035 (2013.01); C07K 2319/04 (2013.01); C07K 2319/21 (2013.01); C07K 2319/35 (2013.01); C07K 2319/41 (2013.01)
Citation (search report)
See references of WO 9856928A1
Designated contracting state (EPC)
DE ES FR GB IT NL
DOCDB simple family (publication)
WO 9856928 A1 19981217; AU 8040798 A 19981230; EP 0980436 A1 20000223
DOCDB simple family (application)
NL 9800335 W 19980608; AU 8040798 A 19980608; EP 98928658 A 19980608