Global Patent Index - EP 0981535 A1

EP 0981535 A1 20000301 - METHODS FOR PRODUCTION AND PURIFICATION OF NUCLEIC ACID MOLECULES

Title (en)

METHODS FOR PRODUCTION AND PURIFICATION OF NUCLEIC ACID MOLECULES

Title (de)

VERFAHREN ZUR HERSTELLUNG UND REINIGUNG VON NUKLEINSÄUREMOLEKÜLEN

Title (fr)

PROCEDES PERMETTANT DE PRODUIRE ET DE PURIFIER LES MOLECULES D'ACIDE NUCLEIQUE

Publication

EP 0981535 A1 20000301 (EN)

Application

EP 98922202 A 19980512

Priority

  • CA 2287525 A 19991112
  • US 9809586 W 19980512
  • US 4621997 P 19970512

Abstract (en)

[origin: WO9851699A1] The present invention is directed to methods for the production and isolation of nucleic acid molecules. In particular, the invention concerns isolation of mRNA molecules and the production and isolation of nucleic acid molecules (e.g., cDNA molecules or libraries), which may be single- or double-stranded. Additionally, the invention concerns selection and isolation of particular nucleic acid molecules of interest from a sample which may contain a population of molecules. Specifically, the invention concerns affinity-labeled primer-adapter molecules which allow improved isolation and production of such nucleic acid molecules, increasing both product recovery and speed of isolation.

IPC 1-7

C07H 21/00; C07H 21/04; C12P 19/34

IPC 8 full level

C07H 21/00 (2006.01); C07H 21/04 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12P 19/34 (2006.01); C12Q 1/68 (2006.01)

CPC (source: EP)

C12N 15/1003 (2013.01); C12N 15/101 (2013.01); C12N 15/1096 (2013.01)

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 9851699 A1 19981119; CA 2287525 A1 20010512; EP 0981535 A1 20000301; EP 0981535 A4 20001129; JP 2002502237 A 20020122

DOCDB simple family (application)

US 9809586 W 19980512; CA 2287525 A 19991112; EP 98922202 A 19980512; JP 54938098 A 19980512