Global patent index - EP 0981635 A1

EP 0981635 A1 2000-03-01 - PRODUCTION OF MATURE PROTEINS IN PLANTS

Title (en)

PRODUCTION OF MATURE PROTEINS IN PLANTS

Title (de)

HERSTELLUNG REIFER PROTEINE IN PFLANZEN

Title (fr)

PRODUCTION DE PROTEINES MATURES DANS DES PLANTES

Publication

EP 0981635 A1 (EN)

Application

EP 98906507 A

Priority

  • US 9803068 W
  • US 3816997 P
  • US 3799197 P
  • US 3817097 P
  • US 3816897 P

Abstract (en)

[origin: WO9836085A1] A method for producing one of the following proteins in transgenic monocot plant cells is disclosed: (i) mature, glycosylated alpha 1-antitrypsin (AAT) having the same N-terminal amino acid sequence as mature AAT produced in humans and a glycosylation pattern which increases serum halflife substantially over that of mature non-glycosylated AAT; (ii) mature, glycosylated antithrombin III (ATIII) having the same N-terminal amino acid sequence as mature ATIII produced in humans; (iii) mature human serum albumin (HSA) having the same N-terminal amino acid sequence as mature HSA produced in humans and having the folding pattern of native mature HSA as evidenced by its bilirubin-binding characteristics; and (iv) mature, active subtilisin BPN' (BPN') having the same N-terminal amino acid sequence as BPN' produced in Bacillus. Monocot plants cells are transformed with a chimeric gene which includes a DNA coding sequence encoding a fusion protein having an (i) N-terminal moiety corresponding to a rice alpha -amylase signal sequence peptide and, (iii) immediately adjacent the C-terminal amino acid of said peptide, a protein moiety corresponding to the mature protein to be produced.

IPC 1-7 (main, further and additional classification)

C12N 15/82; C12N 15/14; C12N 15/15; C12N 15/57; C12P 21/02

IPC 8 full level (invention and additional information)

A01H 5/00 (2006.01); C07K 14/765 (2006.01); C07K 14/81 (2006.01); C12N 5/10 (2006.01); C12N 9/54 (2006.01); C12N 15/09 (2006.01); C12N 15/57 (2006.01); C12N 15/82 (2006.01); C12P 21/02 (2006.01); C12R 1/91 (2006.01)

CPC (invention and additional information)

C07K 14/8128 (2013.01); C07K 14/765 (2013.01); C07K 14/8125 (2013.01); C12N 9/54 (2013.01); C12N 15/8221 (2013.01); C12N 15/8234 (2013.01); C12N 15/8235 (2013.01); C12N 15/8238 (2013.01); C12N 15/8257 (2013.01)

Citation (search report)

See references of WO 9836085A1

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9836085 A1 19980820; AU 6171698 A 19980908; AU 746826 B2 20020502; CA 2280894 A1 19980820; EP 0981635 A1 20000301; JP 2001512318 A 20010821

INPADOC legal status

2003-01-08 [18W] WITHDRAWN

- Ref Legal Event Code: 18W

- Date of withdrawal: 20021107

2002-05-22 [RAX] REQUESTED EXTENSION STATES OF THE EUROPEAN PATENT HAVE CHANGED

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2000-03-01 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 19990913

2000-03-01 [AK] DESIGNATED CONTRACTING STATES:

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- Designated State(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2000-03-01 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO

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