EP 0981635 A1 20000301 - PRODUCTION OF MATURE PROTEINS IN PLANTS
Title (en)
PRODUCTION OF MATURE PROTEINS IN PLANTS
Title (de)
HERSTELLUNG REIFER PROTEINE IN PFLANZEN
Title (fr)
PRODUCTION DE PROTEINES MATURES DANS DES PLANTES
Publication
Application
Priority
- US 9803068 W 19980213
- US 3816997 P 19970213
- US 3799197 P 19970213
- US 3817097 P 19970213
- US 3816897 P 19970213
Abstract (en)
[origin: WO9836085A1] A method for producing one of the following proteins in transgenic monocot plant cells is disclosed: (i) mature, glycosylated alpha 1-antitrypsin (AAT) having the same N-terminal amino acid sequence as mature AAT produced in humans and a glycosylation pattern which increases serum halflife substantially over that of mature non-glycosylated AAT; (ii) mature, glycosylated antithrombin III (ATIII) having the same N-terminal amino acid sequence as mature ATIII produced in humans; (iii) mature human serum albumin (HSA) having the same N-terminal amino acid sequence as mature HSA produced in humans and having the folding pattern of native mature HSA as evidenced by its bilirubin-binding characteristics; and (iv) mature, active subtilisin BPN' (BPN') having the same N-terminal amino acid sequence as BPN' produced in Bacillus. Monocot plants cells are transformed with a chimeric gene which includes a DNA coding sequence encoding a fusion protein having an (i) N-terminal moiety corresponding to a rice alpha -amylase signal sequence peptide and, (iii) immediately adjacent the C-terminal amino acid of said peptide, a protein moiety corresponding to the mature protein to be produced.
IPC 1-7
IPC 8 full level
A01H 5/00 (2006.01); C07K 14/765 (2006.01); C07K 14/81 (2006.01); C12N 5/10 (2006.01); C12N 9/54 (2006.01); C12N 15/09 (2006.01); C12N 15/57 (2006.01); C12N 15/82 (2006.01); C12P 21/02 (2006.01); C12R 1/91 (2006.01)
CPC (source: EP)
C07K 14/765 (2013.01); C07K 14/8125 (2013.01); C07K 14/8128 (2013.01); C12N 9/54 (2013.01); C12N 15/8221 (2013.01); C12N 15/8234 (2013.01); C12N 15/8235 (2013.01); C12N 15/8238 (2013.01); C12N 15/8257 (2013.01)
Citation (search report)
See references of WO 9836085A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9836085 A1 19980820; AU 6171698 A 19980908; AU 746826 B2 20020502; CA 2280894 A1 19980820; EP 0981635 A1 20000301; JP 2001512318 A 20010821
DOCDB simple family (application)
US 9803068 W 19980213; AU 6171698 A 19980213; CA 2280894 A 19980213; EP 98906507 A 19980213; JP 53599798 A 19980213