EP 0983365 A1 20000308 - ENZYME DERIVED FROM THERMOPHILIC ORGANISMS THAT FUNCTIONS AS A CHROMOSOMAL REPLICASE, AND PREPARATION AND USES THEREOF
Title (en)
ENZYME DERIVED FROM THERMOPHILIC ORGANISMS THAT FUNCTIONS AS A CHROMOSOMAL REPLICASE, AND PREPARATION AND USES THEREOF
Title (de)
ALS CHROMOSOMALE REPLICASE FUNKTIONIERENDES ENZYM AUS THERMOPHILEN ORGANISMEN UND SEINE ANWENDUNG
Title (fr)
ENZYME DERIVEE D'ORGANISMES THERMOPHILES FONCTIONNANT COMME REPLICASE CHROMOSOMIQUE, PRODUCTION ET EMPLOIS DE CETTE ENZYME
Publication
Application
Priority
- US 9806921 W 19980408
- US 82340797 A 19970408
Abstract (en)
[origin: WO9845452A2] A DNA Polymerase has been identified in a thermophile that functions as a chromosomal replicase. The specific enzyme is a holoenzyme III that has been identified in Thermus thermophilus, and corresponds to Polymerase III in E. coli. The genes and the polypeptides corresponding to T.th.. gamma , tau , epsilon , alpha and beta subunits that they encode are disclosed, as are probes, vectors, methods of preparation and the methods of use. The enzymes of the present invention and their components are particularly well suited for use in procedures for the preparation of DNA, such as PCR, because of the speed and accuracy that they are able to achieve.
IPC 1-7
IPC 8 full level
C12N 1/21 (2006.01); C12N 9/12 (2006.01); C12N 15/54 (2006.01)
CPC (source: EP)
C12N 9/1252 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9845452 A2 19981015; WO 9845452 A3 19981217; AU 7683998 A 19981030; EP 0983365 A1 20000308
DOCDB simple family (application)
US 9806921 W 19980408; AU 7683998 A 19980408; EP 98924742 A 19980408