Global Patent Index - EP 0983365 A1

EP 0983365 A1 2000-03-08 - ENZYME DERIVED FROM THERMOPHILIC ORGANISMS THAT FUNCTIONS AS A CHROMOSOMAL REPLICASE, AND PREPARATION AND USES THEREOF

Title (en)

ENZYME DERIVED FROM THERMOPHILIC ORGANISMS THAT FUNCTIONS AS A CHROMOSOMAL REPLICASE, AND PREPARATION AND USES THEREOF

Title (de)

ALS CHROMOSOMALE REPLICASE FUNKTIONIERENDES ENZYM AUS THERMOPHILEN ORGANISMEN UND SEINE ANWENDUNG

Title (fr)

ENZYME DERIVEE D'ORGANISMES THERMOPHILES FONCTIONNANT COMME REPLICASE CHROMOSOMIQUE, PRODUCTION ET EMPLOIS DE CETTE ENZYME

Publication

EP 0983365 A1 (EN)

Application

EP 98924742 A

Priority

  • US 9806921 W
  • US 82340797 A

Abstract (en)

[origin: WO9845452A2] A DNA Polymerase has been identified in a thermophile that functions as a chromosomal replicase. The specific enzyme is a holoenzyme III that has been identified in Thermus thermophilus, and corresponds to Polymerase III in E. coli. The genes and the polypeptides corresponding to T.th.. gamma , tau , epsilon , alpha and beta subunits that they encode are disclosed, as are probes, vectors, methods of preparation and the methods of use. The enzymes of the present invention and their components are particularly well suited for use in procedures for the preparation of DNA, such as PCR, because of the speed and accuracy that they are able to achieve.

IPC 1-7 (main, further and additional classification)

C12N 15/54; C12N 1/21; C12N 15/10; C12N 15/70; C12Q 1/68

IPC 8 full level (invention and additional information)

C12N 1/21 (2006.01); C12N 9/12 (2006.01); C12N 15/54 (2006.01)

CPC (invention and additional information)

C12N 9/1252 (2013.01)

Citation (search report)

See references of WO 9845452A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9845452 A2 19981015; WO 9845452 A3 19981217; AU 7683998 A 19981030; EP 0983365 A1 20000308

INPADOC legal status


2002-05-29 [18W] WITHDRAWN

- Date of withdrawal: 20020327

2000-03-08 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 19991108

2000-03-08 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE