EP 0988390 A1 20000329 - ALTERNATIVELY TARGETED ADENOVIRUS
Title (en)
ALTERNATIVELY TARGETED ADENOVIRUS
Title (de)
ADENOVIRUS MIT VERÄNDERTER ZIELZELLENSPEZIFITÄT
Title (fr)
ADENOVIRUS A CIBLAGE ALTERNATIF
Publication
Application
Priority
- US 9811024 W 19980528
- US 4784997 P 19970528
- US 7166898 P 19980116
Abstract (en)
[origin: WO9854346A1] The present invention provides a trimer comprising three monomers, each having an amino terminus of an adenoviral fiber protein and each having a trimerization domain. The trimer exhibits reduced affinity for a native substrate than a native adenoviral fiber trimer. The present invention further provides an adenovirus incorporating the trimer of the present invention. The present invention also provides a cell line expressing a non-native cell-surface receptor to which an adenovirus having a ligand for the receptor binds, and a method of propagating an adenovirus using the cell line. The present invention also provides a method of purifying an adenovirus having a ligand for a substrate from a composition comprising the adenovirus. The method involves exposing the composition to the substrate under conditions to promote the ligand to selectively bind the substrate. Subsequently, the composition not bound to the substrate is separated from the substrate, after which the bound adenovirus is eluted from the substrate. The present invention further provides a method of inactivating an adenovirus having a ligand recognizing a blood- or lymph-borne substrate by exposing the virus to the substrate. Within the blood or lymph, the ligand binds its substrate, thereby adsorbing the free virus from the blood or lymph. Additionally, the present invention provides a chimeric blocking protein comprising a substrate for an adenovirus fiber, and a method of interfering with adenoviral receptor binding by incubating an adenovirus with such chimeric blocking protein in a solution such that the chimeric blocking protein binds the fiber.
IPC 1-7
IPC 8 full level
A61K 35/76 (2006.01); A61K 48/00 (2006.01); C07K 14/075 (2006.01); C07K 14/705 (2006.01); C07K 19/00 (2006.01); C12N 15/09 (2006.01); C12N 7/00 (2006.01); C12N 15/861 (2006.01); C12P 21/02 (2006.01); C12R 1/91 (2006.01)
CPC (source: EP)
C07K 14/005 (2013.01); C12N 15/86 (2013.01); C07K 2319/00 (2013.01); C12N 2710/10322 (2013.01); C12N 2710/10343 (2013.01); C12N 2810/405 (2013.01); C12N 2810/855 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9854346 A1 19981203; BR 9809173 A 20000801; CA 2291323 A1 19981203; EP 0988390 A1 20000329; HU P0002070 A2 20001028; IL 133010 A0 20010319; JP 2001518806 A 20011016; NZ 501140 A 20010727; PL 337130 A1 20000731; SK 159999 A3 20000612
DOCDB simple family (application)
US 9811024 W 19980528; BR 9809173 A 19980528; CA 2291323 A 19980528; EP 98923856 A 19980528; HU P0002070 A 19980528; IL 13301098 A 19980528; JP 50097799 A 19980528; NZ 50114098 A 19980528; PL 33713098 A 19980528; SK 159999 A 19980528