Global patent index - EP 0988395 A2

EP 0988395 A2 2000-03-29 - ASSAY METHODS AND COMPOSITIONS USEFUL FOR MEASURING RECEPTOR LIGAND BINDING

Title (en)

ASSAY METHODS AND COMPOSITIONS USEFUL FOR MEASURING RECEPTOR LIGAND BINDING

Title (de)

NACHWEISVERFAHREN UND ZUSAMMENSETZUNGEN UM REZEPTOR-LIGAND BINDUNGEN ZU MESSEN

Title (fr)

METHODES ET COMPOSITIONS D'ANALYSE UTILES POUR EVALUER LA LIAISON D'UN LIGAND A SON RECEPTEUR

Publication

EP 0988395 A2 (EN)

Application

EP 98929167 A

Priority

  • CA 9800581 W
  • US 87466397 A

Abstract (en)

[origin: WO9858074A2] This invention provides a system for screening chemical compounds to identify ligands for receptors including G-protein coupled receptors. The invention exploits cells in which the receptor is coupled through a second messenger system to an ion channel that is gated by cyclic nucleotide. Receptor stimulation causes the second messenger system to produce cyclic nucleotide, which results in ion influx through the channel. By measuring ion influx fluorescently, the invention provides a rapid and convenient means for identifying receptor ligands. By providing mixed cell cultures that include cells expressing different receptor types, and by loading into those cells different fluorescent reporters of ion influx, the invention further provides a multiplexed system that accelerates the ligand identification process. Cells useful in the process, and mehtods for exploiting them, are described.

IPC 1-7 (main, further and additional classification)

C12Q 1/00

IPC 8 full level (invention and additional information)

C07K 14/705 (2006.01); C07K 14/72 (2006.01); G01N 33/566 (2006.01); G01N 33/94 (2006.01)

CPC (invention and additional information)

C07K 14/705 (2013.01); C07K 14/723 (2013.01); G01N 33/566 (2013.01); G01N 33/9413 (2013.01); G01N 33/942 (2013.01); G01N 2333/726 (2013.01); G01N 2500/00 (2013.01)

Citation (search report)

See references of WO 9858074A3

Designated contracting state (EPC)

BE CH DE DK FR GB IT LI NL SE

EPO simple patent family

WO 9858074 A2 19981223; WO 9858074 A3 19990401; AU 7902398 A 19990104; CA 2293254 A1 19981223; EP 0988395 A2 20000329

INPADOC legal status

2004-07-14 [18D] DEEMED TO BE WITHDRAWN

- Ref Legal Event Code: 18D

- Effective date: 20040101

2000-03-29 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 19991213

2000-03-29 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): BE CH DE DK FR GB IT LI NL SE