EP 0988531 A1 20000329 - METHOD FOR IDENTIFYING AND/OR ANALYSING BIOLOGICAL SUBSTANCES, PRESENT IN A CONDUCTIVE LIQUID, DEVICE AND AFFINITY SENSOR USED FOR IMPLEMENTING SAID METHOD
Title (en)
METHOD FOR IDENTIFYING AND/OR ANALYSING BIOLOGICAL SUBSTANCES, PRESENT IN A CONDUCTIVE LIQUID, DEVICE AND AFFINITY SENSOR USED FOR IMPLEMENTING SAID METHOD
Title (de)
VERFAHREN, VORRICHTUNG UND AFFINITÄTSSENSOR ZUR IDENTIFIZIERUNG UND/ODER DOSIERUNG VON BIOLOGISCHEN SUBSTANZEN IN EINER LEITENDEN FLÜSSIGKEIT
Title (fr)
PROCEDE D'IDENTIFICATION ET/OU DE DOSAGE DE SUBSTANCES BIOLOGIQUES, PRESENTES DANS UN LIQUIDE CONDUCTEUR, DISPOSITIF ET CAPTEUR D'AFFINITE UTILES POUR LA MISE EN OEUVRE DE CE PROCEDE
Publication
Application
Priority
- FR 9801213 W 19980611
- FR 9707530 A 19970611
Abstract (en)
[origin: FR2764702A1] The invention concerns an improved method for identifying and/or analysing polynucleotides PN present in a conductive liquid medium, using an affinity sensor comprising a multilayer structure semiconductor/insulant/probe = polynucleotides PNc complementary to the medium PN. The method consists in selecting non-marked PNc; polarising the semiconductor; periodically lighting it; measuring the flat band potential variations induced by loading effect phenomenon directly and essentially related to specific pairings between PN and PNc; and finally interpreting the signals collected at the end of the identification and/or analysis of PN. The invention also concerns the device and the sensor used for implementing the method. It is applicable to molecular biology analysis, e.g. diagnosis of viral or genetic diseases.
IPC 1-7
IPC 8 full level
C12Q 1/68 (2006.01); C12Q 1/6825 (2018.01); G01N 27/30 (2006.01); G01N 27/416 (2006.01); G01N 33/543 (2006.01)
CPC (source: EP US)
C12Q 1/6825 (2013.01 - EP US); G01N 27/305 (2013.01 - EP US); G01N 33/5438 (2013.01 - EP US)
Citation (search report)
See references of WO 9857157A1
Designated contracting state (EPC)
DE FR GB IT
DOCDB simple family (publication)
FR 2764702 A1 19981218; FR 2764702 B1 19990903; AU 8112898 A 19981230; CA 2294627 A1 19981217; CA 2294627 C 20090609; EP 0988531 A1 20000329; JP 2002503343 A 20020129; US 6475728 B1 20021105; WO 9857157 A1 19981217
DOCDB simple family (application)
FR 9707530 A 19970611; AU 8112898 A 19980611; CA 2294627 A 19980611; EP 98930829 A 19980611; FR 9801213 W 19980611; JP 50178699 A 19980611; US 42487700 A 20000331