EP 0990051 A1 20000405 - IDENTIFICATION OF MOLECULAR TARGETS
Title (en)
IDENTIFICATION OF MOLECULAR TARGETS
Title (de)
IDENTIFIKATION VON MOLEKULARTARGETS
Title (fr)
IDENTIFICATION DE CIBLES MOLECULAIRES
Publication
Application
Priority
- US 9814082 W 19980707
- US 5178097 P 19970707
- US 5178197 P 19970707
Abstract (en)
[origin: WO9902733A1] Identification of the molecular targets of a drug or toxin is the first step in understanding how the drug or toxin works, and important advance in learning how to improve a drug or assess the risks due to a toxin. The primary action of a drug usually involves binding to a protein; secondary actions may express themselves in the form of side effects and in some cases may be due to binding to other proteins. Consequently, it is useful to identify all physiologically relevant sites of action of a drug or toxin. A simple method for obtaining a list of the potential targets of a drug, toxin or other biologically active substance (referred to collectively as ligands) involves a multistep process. The first step is screeening a protein or peptide library to identify library members that exhibit high affinity for a particular ligand. The second step involves searching of sequence data bases for proteins that contain the sequences of the library members shown to have high affinity for the ligand. The proteins thus identified constitute a list of potential targets for the ligand. If random peptide libraries have been used, the position of identified consensus sequences within the identified protein constitutes an identification of the potential ligand binding site on the target.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2006.01); C40B 40/02 (2006.01); G01N 33/566 (2006.01); G01N 33/68 (2006.01)
CPC (source: EP)
C12N 15/1037 (2013.01); C12Q 1/6811 (2013.01); C40B 40/02 (2013.01); G01N 33/68 (2013.01)
Citation (search report)
See references of WO 9902733A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9902733 A1 19990121; EP 0990051 A1 20000405; JP 2003524364 A 20030819
DOCDB simple family (application)
US 9814082 W 19980707; EP 98945752 A 19980707; JP 2000502224 A 19980707