Global Patent Index - EP 0990149 A1

EP 0990149 A1 2000-04-05 - NUCLEIC ACID SENSOR

Title (en)

NUCLEIC ACID SENSOR

Title (de)

NUKLEINSÄURE-SENSOR

Title (fr)

CAPTEUR D'ACIDE NUCLEIQUE

Publication

EP 0990149 A1 (EN)

Application

EP 97921532 A

Priority

  • AU 9700316 W
  • US 1812596 P

Abstract (en)

[origin: WO9744651A1] The present invention provides biosensors and methods for the detection selected nucleic acid sequences. In one form the biosensor comprises an electrode and a bilayer membrane having a top and a bottom layer. The bottom layer being proximal to and connected to the electrode in a manner such that a space exists between the membrane and the electrode, with the conductance or impedance of the membrane being dependent on the presence or absence of the selected nucleic acid sequence. The membrane comprises a closely packed array of amphiphilic molecules and a plurality of ion channels comprising first half membrane spanning monomers dispersed in the top layer and second half membrane spanning monomers dispersed in the bottom layer. The first half membrane spanning monomers are capable of lateral diffusion within the upper layer and the second half membrane-spanning monomers are prevented from lateral diffusion within the bottom layer. A first ligand specifically reactive with the selected nucleic acid sequence is attached to an end of a proportion of the first half membrane-spanning monomers proximal the surface of the membrane and a second ligand reactive with the selected nucleic acid or marker attached thereto is attached to an end of the remainder of the first half membrane-spanning monomers proximal the surface of the membrane.

IPC 1-7 (main, further and additional classification)

G01N 27/327; G01N 27/333; G01N 27/40; G01N 33/53; G01N 33/553; G01N 33/569

IPC 8 full level (invention and additional information)

G01N 33/50 (2006.01); C12Q 1/68 (2006.01); G01N 27/327 (2006.01); G01N 27/333 (2006.01)

CPC (invention and additional information)

C12Q 1/6825 (2013.01)

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9744651 A1 19971127; AU 2757897 A 19971209; AU 734086 B2 20010531; CA 2255952 A1 19971127; EP 0990149 A1 20000405; EP 0990149 A4 20030226; JP 2000513811 A 20001017

INPADOC legal status


2003-07-09 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20021202

2003-02-26 [A4] SUPPLEMENTARY SEARCH REPORT

- Effective date: 20030113

2003-02-26 [RIC1] CLASSIFICATION (CORRECTION)

- IPC: 7G 01N 27/327 A

2003-02-26 [RIC1] CLASSIFICATION (CORRECTION)

- IPC: 7G 01N 27/333 B

2003-02-26 [RIC1] CLASSIFICATION (CORRECTION)

- IPC: 7G 01N 27/40 B

2003-02-26 [RIC1] CLASSIFICATION (CORRECTION)

- IPC: 7G 01N 33/53 B

2003-02-26 [RIC1] CLASSIFICATION (CORRECTION)

- IPC: 7G 01N 33/553 B

2003-02-26 [RIC1] CLASSIFICATION (CORRECTION)

- IPC: 7G 01N 33/569 B

2003-02-26 [RIC1] CLASSIFICATION (CORRECTION)

- IPC: 7C 12Q 1/68 B

2003-02-26 [RIC1] CLASSIFICATION (CORRECTION)

- IPC: 7G 01N 27/403 B

2000-04-05 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 19981210

2000-04-05 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE