EP 0994188 B1 20040107 - Method for obtaining active beta-NGF
Title (en)
Method for obtaining active beta-NGF
Title (de)
Verfahren zur Gewinnung von aktivem Beta-NGF
Title (fr)
Procédé d'obtention du NGF-bêta biologiquement actif
Publication
Application
Priority
EP 98119077 A 19981009
Abstract (en)
[origin: EP0994188A1] Preparation of biologically active beta-NGF comprises denaturing its inactive insoluble proform followed by renaturation and pro-sequence cleavage. Preparation of biologically active beta -NGF from its inactive insoluble proform, obtained by recombinant preparation in prokaryotic cells comprises: (a) dissolving ProNGF in its inactive insoluble form in a denaturating solution ; (b) transferring into a non- or weak denaturing solution where the denatured proNGF assumes a biologically active conformation, determined by the presence of disulfide bridges occurring in natural beta -NGF; and (c) cleaving the pro-sequence to obtain the active beta -NGF followed by its isolation.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); A61K 38/00 (2006.01); C07K 1/107 (2006.01); C07K 1/12 (2006.01); C07K 14/48 (2006.01); C12N 15/12 (2006.01); C12P 21/06 (2006.01)
CPC (source: EP KR US)
A61P 25/00 (2017.12 - EP); A61P 25/02 (2017.12 - EP); A61P 25/28 (2017.12 - EP); C07K 14/48 (2013.01 - EP KR US)
Citation (examination)
WO 9608562 A1 19960321 - MAX PLANCK GESELLSCHAFT [DE], et al
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB IE IT LI LU NL PT SE
DOCDB simple family (publication)
EP 0994188 A1 20000419; EP 0994188 B1 20040107; AT E257514 T1 20040115; AU 1034800 A 20000501; AU 765810 B2 20031002; BR 9914393 A 20010626; BR 9914393 B1 20210622; CA 2346257 A1 20000420; CA 2346257 C 20050301; DE 59810555 D1 20040212; ES 2213861 T3 20040901; FR 17C0007 I1 20171117; FR 17C0007 I2 20181109; JP 2002527062 A 20020827; JP 2010280717 A 20101216; JP 4634611 B2 20110216; JP 5275306 B2 20130828; KR 100593831 B1 20060628; KR 20010083900 A 20010903; NL 300894 I2 20180201; US 2010203589 A1 20100812; US 8318671 B1 20121127; US 8501439 B2 20130806; WO 0022119 A1 20000420; ZA 200102780 B 20020704
DOCDB simple family (application)
EP 98119077 A 19981009; AT 98119077 T 19981009; AU 1034800 A 19991011; BR 9914393 A 19991011; CA 2346257 A 19991011; DE 59810555 T 19981009; EP 9907613 W 19991011; ES 98119077 T 19981009; FR 17C0007 C 20170927; JP 2000576009 A 19991011; JP 2010199387 A 20100906; KR 20017004512 A 20010409; NL 300894 C 20170928; US 76522910 A 20100422; US 80709699 A 19991011; ZA 200102780 A 20010404