Global Patent Index - EP 0996631 A1

EP 0996631 A1 2000-05-03 - MODIFIED NUCLEIC ACID ANALOGUES

Title (en)

MODIFIED NUCLEIC ACID ANALOGUES

Title (de)

MODIFIZIERTE NUKLEINSÄUREANALOGA

Title (fr)

ANALOGUES D'ACIDE NUCLEIQUE MODIFIES

Publication

EP 0996631 A1 (DE)

Application

EP 98966833 A

Priority

  • DE 19757516 A
  • EP 9808317 W

Abstract (en)

[origin: DE19757516A1] A molecule that hybridises with nucleic acids and has a backbone of linearly linked monomer units, where at least one of the monomer units is a non-nucleoside unit containing an acid function with a pKa of less than 3.0, is new. Independent claims are also included for the following: (1) a molecule of formula (IV): where L = H, OH, 1-4C alkanoyl, an optionally protected nucleobase-binding group, 6-14C aryl, N-, O- and/or S-containing heterocyclyl, or an intercalator or reporter group; A = a spacer of 1-3 atoms; x = 0-3; E and F = COOX, CSOX, SO2X, SO3X or NR<1>Y; R<1> = H or 1-3C alkyl; X = H or a protecting/activating group; Y = H or a protecting group; N = amine nitrogen; C and D = (CR<2>R<3>)n or CR<2>R<3>CR<4>R<5>; R<2>-R<5> = H, optionally substituted 1-6C alkyl, OH, COOH or an optionally protected group containing an acid function with a pKa of less than 3.0; n = 1-4; (2) a method for detecting the presence or absence of a nucleic acid in a sample suspected of containing one or more other nucleic acids, whose base sequence differs from that of the target nucleic acid in one or more positions, comprising contacting the sample with a probe whose base sequence is complementary to the target sequence but not to the base sequence of the other nucleic acids and determining if the probe has bound to its complementary base sequence and the probe is a molecule in which fewer than 80% but at least one of its bases are bonded to negatively charged backbone units; (3) use of negative charges in probes containing at least one non-nucleoside backbone unit to avoid nonspecific binding of the probes to (a) base sequences that are less than 50% complementary to the probes or (b) solid supports; (4) use of acid functions to increase the selectivity of probes in binding reactions; (5) use of molecules that hybridize with nucleic acids and have at least one base bonded to a negatively charged non-nucleoside backbone unit as primers for polymerase-catalysed extension with the aid of mononucleoside triphosphates; and (6) use of molecules that bind to nucleic acids and have at least one base bonded to a negatively charged non-nucleoside backbone unit for transfection into cells with the aid of transfection reagents.

IPC 1-7 (main, further and additional classification)

C07K 14/00; C07H 21/00; C12Q 1/68

IPC 8 full level (invention and additional information)

C07H 21/00 (2006.01); C07K 14/00 (2006.01); C12Q 1/68 (2006.01)

CPC (invention and additional information)

C07K 14/003 (2013.01); C07H 21/00 (2013.01); C12Q 1/6832 (2013.01); Y02P 20/55 (2015.11)

Combination set (CPC)

C12Q 1/6832 + C12Q 2563/173 + C12Q 2527/119 + C12Q 2525/113

Citation (search report)

See references of WO 9933867A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

DE 19757516 A1 19990624; EP 0996631 A1 20000503; WO 9933867 A2 19990708; WO 9933867 A3 19990902

INPADOC legal status


2002-11-06 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20020425

2001-11-28 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20011015

2000-07-12 [RAP1] TRANSFER OF RIGHTS OF AN EP PUBLISHED APPLICATION

- Owner name: DAKO A/S

2000-05-03 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000127

2000-05-03 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE