EP 0996742 A1 20000503 - METHODS FOR CLEAVING DNA WITH NUCLEOTIDE INTEGRASES
Title (en)
METHODS FOR CLEAVING DNA WITH NUCLEOTIDE INTEGRASES
Title (de)
VERFAHREN ZUR SPALTUNG VON DNA MITTELS NUKLEOTID-INTEGRASEN
Title (fr)
PROCEDE DE CLIVAGE D'ADN AU MOYEN D'INTEGRASES NUCLEOTIDIQUES
Publication
Application
Priority
- US 9803990 W 19980227
- US 3922097 P 19970228
- US 7616198 P 19980226
Abstract (en)
[origin: CA2282737A1] The present invention provides methods, using a nucleotide integrase, for cleaving nucleic acids substrates at specific sites and inserting a nucleic acid molecule into the cleaved substrate. The method of cleaving one strand of a double-stranded DNA substrate comprises providing a nucleotide integrase comprising a group II-intron RNA having two hybridizing sequences capable of hybridizing with two intron RNA binding sequences on the one strand of the substrate and a group II-intron encoded protein which binds to a first sequence element of the substrate. The method of cleaving both strands of a double-stranded DNA substrate comprises providing a nucleotide integrase comprising a group II-intron RNA having two hybridizing sequences capable of hybridizing with two intron RNA binding sequences on one strand of the substrate and a group II-intron encoded protein capable of binding to first and second sequence elements in the recognition site of the substrate. The method of cleaving a single-stranded nucleic acid substrate comprises providing an integrase having two hybridizing sequences capable of hybridizing with two intron RNA-binding sequences of the substrate and a group II-intron encoded protein.
IPC 1-7
IPC 8 full level
C12N 15/10 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
C12N 15/10 (2013.01); C12Q 1/683 (2013.01)
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB IT LI NL SE
DOCDB simple family (publication)
AU 6676298 A 19980918; AU 748237 B2 20020530; CA 2282737 A1 19980903; EP 0996742 A1 20000503; EP 0996742 A4 20060208
DOCDB simple family (application)
AU 6676298 A 19980227; CA 2282737 A 19980227; EP 98908823 A 19980227