Global Patent Index - EP 0998574 A1

EP 0998574 A1 2000-05-10 - METHODS OF PERFORMING HOMOLOGOUS RECOMBINATION BASED MODIFICATION OF NUCLEIC ACIDS IN RECOMBINATION DEFICIENT CELLS AND USE OF THE MODIFIED NUCLEIC ACIDS

Title (en)

METHODS OF PERFORMING HOMOLOGOUS RECOMBINATION BASED MODIFICATION OF NUCLEIC ACIDS IN RECOMBINATION DEFICIENT CELLS AND USE OF THE MODIFIED NUCLEIC ACIDS

Title (de)

METHODEN FÜR DIE AUSFÜHRUNG VON HOMOLOGER REKOMBINATION ZUR MODIFIKATION VON NUKLEINSÄUREN IN REKOMBINATIONSDEFIZIENTEN ZELLEN UND DIE VERWENDUNG DER MODIFIZIERTEN NUKLEINSÄUREN

Title (fr)

PROCEDES DE PREFORMAGE DE RECOMBINAISON HOMOLOGUE D'APRES UNE MODIFICATION D'ACIDES NUCLEIQUES DANS DES CELLULES PRESENTANT UNE DEFICIENCE DE RECOMBINAISON, ET UTILISATION DES PRODUITS D'ACIDES NUCLEIQUES MODIFIES DE CELLES-CI

Publication

EP 0998574 A1 (EN)

Application

EP 98930459 A

Priority

  • US 9812966 W
  • US 88096697 A
  • US 10249098 A

Abstract (en)

[origin: WO9859060A1] A simple method for modifying genes in a recombination deficient host cell is disclosed. Such modifications include generating insertion, deletions, substitutions, and/or point mutations at any chosen site in the independent origin based cloning vector. The modified gene can be contained in an independent origin based cloning vector that is used to introduce a modified heterologous gene into a cell. Such a modified vector may be used in the production of a germline transmitted transgenic animal, or in gene targeting protocols in eukaryotic cells.

IPC 1-7 (main, further and additional classification)

C12N 15/70; A01K 67/027; C12N 15/90

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); A01K 67/027 (2006.01); C12N 15/70 (2006.01); C12N 15/90 (2006.01)

CPC (invention and additional information)

A01K 67/0275 (2013.01); C12N 15/70 (2013.01); C12N 15/902 (2013.01); A01K 2217/05 (2013.01); C12N 2800/202 (2013.01); C12N 2800/204 (2013.01); C12N 2840/203 (2013.01)

Citation (search report)

See references of WO 9859060A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9859060 A1 19981230; AU 730859 B2 20010315; AU 7984898 A 19990104; CA 2294619 A1 19981230; EP 0998574 A1 20000510; JP 2002515764 A 20020528

INPADOC legal status


2003-12-17 [18D] APPLICATION DEEMED TO BE WITHDRAWN

- Effective date: 20030615

2003-03-19 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20030204

2000-05-10 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000124

2000-05-10 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2000-05-10 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO:

- Free text: AL PAYMENT 20000124;LT PAYMENT 20000124;LV PAYMENT 20000124;MK PAYMENT 20000124;RO PAYMENT 20000124;SI PAYMENT 20000124