EP 1000139 A1 20000517 - CULTURING APPARATUS AND CULTIVATING METHOD
Title (en)
CULTURING APPARATUS AND CULTIVATING METHOD
Title (de)
KULTURVORRICHTUNG UND KULTIVATIONSVERFAHREN
Title (fr)
APPAREIL ET PROCEDE DE CULTURE
Publication
Application
Priority
- US 9908552 W 19990419
- US 8233298 P 19980420
Abstract (en)
[origin: WO9954433A1] Disclosed is a culturing apparatus (100) that includes a gas-permeable solid phase culturing bed (110) disposed within an interior space of a vessel (108). The culturing bed comprises a solid substrate that includes a biological agent disposed thereon. A gas inlet (115, 116) permits selective introduction of gas into the vessel (108) under pressure sufficient to cause gas to flow through at least a portion of the bed (110). Also disclosed is a method of cultivating a biological agent. The method comprises providing a gas-permeable culturing bed, flowing a gas through the bed and recovering at least a portion of the biological agent. The gas preferably is an oxygen-containing gas, whereby the flow of gas through the bed provides convective mass transfer of oxygen to the biological agent. The apparatus and method of the invention permit "pressure pulsing" of gas through the bed.
IPC 1-7
IPC 8 full level
C12M 1/04 (2006.01); C12M 1/16 (2006.01); C12M 1/38 (2006.01)
CPC (source: EP)
C12M 25/18 (2013.01); C12M 29/14 (2013.01); C12M 41/40 (2013.01)
Citation (search report)
See references of WO 9954433A1
Designated contracting state (EPC)
ES FR IE NL
DOCDB simple family (publication)
WO 9954433 A1 19991028; AU 3652899 A 19991108; BR 9906365 A 20000919; CA 2294901 A1 19991028; EP 1000139 A1 20000517; JP 2002506356 A 20020226
DOCDB simple family (application)
US 9908552 W 19990419; AU 3652899 A 19990419; BR 9906365 A 19990419; CA 2294901 A 19990419; EP 99918670 A 19990419; JP 55321399 A 19990419