Global Patent Index - EP 1000142 A1

EP 1000142 A1 2000-05-17 - METHODS FOR SCREENING BACTERIOPHAGE LIBRARIES

Title (en)

METHODS FOR SCREENING BACTERIOPHAGE LIBRARIES

Title (de)

VERFAHREN ZUR SICHTUNG VON PHAGENBIBLIOTHEKEN

Title (fr)

PROCEDE DE CRIBLAGE DE BIBLIOTHEQUES DE BACTERIOPHAGES

Publication

EP 1000142 A1 (EN)

Application

EP 98936536 A

Priority

  • GB 9802269 W
  • GB 9716094 A

Abstract (en)

[origin: WO9906542A1] A method is provided for producing a modified bacteriophage library suitable for use in combination with cells of a selected strain that have been transformed for expression of a heterologous (non native) protein in a screening procedure in which specific binding between an individual bacteriophage of the library and a recognition site of the heterologous protein is detected. The process comprises: (a) subjecting an initial library containing said individual bacteriophage and other bacteriophage to a fractionation procedure which comprises contacting the initial bacteriophage library with cells of said selected strain which do not express said heterologous protein, whereby members of the bacteriophage library which bind to one or more proteins other than said heterologous protein are bound to the cells; (b) separating bound and unbound bacteriophage; and (c) isolating a modified bacteriophage library depleted in library members which bind to one or more proteins other than said heterologous protein. The modified bacteriophage libraries produced by the method, peptide ligands identified and/or isolated by screening procedures using the modified libraries and the use of identified ligands and consensus sequences thereof in the design of pharmaceutical and diagnostic agents also form part of the invention.

IPC 1-7 (main, further and additional classification)

C12N 15/10; C12Q 1/02

IPC 8 full level (invention and additional information)

C12N 15/10 (2006.01); C12Q 1/02 (2006.01); C40B 40/02 (2006.01)

CPC (invention and additional information)

C40B 40/02 (2013.01); C12N 15/1037 (2013.01); C12Q 1/025 (2013.01)

Citation (search report)

See references of WO 9906542A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9906542 A1 19990211; AU 8550398 A 19990222; EP 1000142 A1 20000517; GB 9716094 D0 19971001

INPADOC legal status


2002-11-27 [18W] WITHDRAWN

- Date of withdrawal: 20021001

2000-05-17 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000214

2000-05-17 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2000-05-17 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO

- Free text: AL PAYMENT 20000214;LT PAYMENT 20000214;LV PAYMENT 20000214;MK PAYMENT 20000214;RO PAYMENT 20000214;SI PAYMENT 20000214