EP 1000142 A1 20000517 - METHODS FOR SCREENING BACTERIOPHAGE LIBRARIES
Title (en)
METHODS FOR SCREENING BACTERIOPHAGE LIBRARIES
Title (de)
VERFAHREN ZUR SICHTUNG VON PHAGENBIBLIOTHEKEN
Title (fr)
PROCEDE DE CRIBLAGE DE BIBLIOTHEQUES DE BACTERIOPHAGES
Publication
Application
Priority
- GB 9802269 W 19980729
- GB 9716094 A 19970730
Abstract (en)
[origin: WO9906542A1] A method is provided for producing a modified bacteriophage library suitable for use in combination with cells of a selected strain that have been transformed for expression of a heterologous (non native) protein in a screening procedure in which specific binding between an individual bacteriophage of the library and a recognition site of the heterologous protein is detected. The process comprises: (a) subjecting an initial library containing said individual bacteriophage and other bacteriophage to a fractionation procedure which comprises contacting the initial bacteriophage library with cells of said selected strain which do not express said heterologous protein, whereby members of the bacteriophage library which bind to one or more proteins other than said heterologous protein are bound to the cells; (b) separating bound and unbound bacteriophage; and (c) isolating a modified bacteriophage library depleted in library members which bind to one or more proteins other than said heterologous protein. The modified bacteriophage libraries produced by the method, peptide ligands identified and/or isolated by screening procedures using the modified libraries and the use of identified ligands and consensus sequences thereof in the design of pharmaceutical and diagnostic agents also form part of the invention.
IPC 1-7
IPC 8 full level
C12N 15/10 (2006.01); C12Q 1/02 (2006.01); C40B 40/02 (2006.01)
CPC (source: EP)
C12N 15/1037 (2013.01); C12Q 1/025 (2013.01); C40B 40/02 (2013.01)
Citation (search report)
See references of WO 9906542A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9906542 A1 19990211; AU 8550398 A 19990222; EP 1000142 A1 20000517; GB 9716094 D0 19971001
DOCDB simple family (application)
GB 9802269 W 19980729; AU 8550398 A 19980729; EP 98936536 A 19980729; GB 9716094 A 19970730