Global Patent Index - EP 1002053 A1

EP 1002053 A1 2000-05-24 - MEDIUM FOR RECOVERY AND GROWTH OF MICROORGANISMS

Title (en)

MEDIUM FOR RECOVERY AND GROWTH OF MICROORGANISMS

Title (de)

MEDIUM ZUR ERHOLUNG UND WACHSTUM VON MICROORGANISMEN

Title (fr)

SUPPORT DE RECUPERATION ET DE CULTURE DE MICRO-ORGANISMES

Publication

EP 1002053 A1 (EN)

Application

EP 98932405 A

Priority

  • EP 98932405 A
  • EP 97307332 A
  • GB 9802015 W
  • GB 9714594 A
  • GB 9721396 A

Abstract (en)

[origin: WO9902650A1] A method of enriching the population of a target microorganism in a sample, comprises incubating the sample in a pre-enrichment medium, with one or more selective agents to favour growth of the target microorganism arranged for release into the medium after a predetermined time delay. The method of the invention combines pre-enrichment and selective enrichment steps by use of timed release of the selective agent(s). This has the benefits of reducing the amount of manipulation and labour necessary for these stages, and also of reducing the amount of time required for these stages. It has been found that these stages can be satisfactorily completed within 24 hours, without the need for operator intervention. The enrichment can be followed by identification of the target microorganism, using any appropriate technique, either conventional or rapid. Using rapid identification techniques, total test time can be reduced to a little over 24 hours. The invention finds particular application in the analysis of samples of foodstuffs and beverages in the enrichment and identification of a wide range of foodborne pathogens including Salmonella, Listeria, Campylobacter and E. coli 0157, but can also be used in connection with a wider range of target organisms from a much wider range of sample types including environmental samples.

IPC 1-7 (main, further and additional classification)

C12N 1/20; C12M 1/04; C12N 1/38

IPC 8 full level (invention and additional information)

C12M 1/04 (2006.01); C12N 1/20 (2006.01); C12N 1/38 (2006.01); C12Q 1/02 (2006.01); C12Q 1/04 (2006.01); C12Q 1/25 (2006.01)

CPC (invention and additional information)

C12Q 1/02 (2013.01); C12N 1/20 (2013.01); C12Q 1/25 (2013.01)

Citation (search report)

See references of WO 9902649A1

Designated contracting state (EPC)

DE FR GB IT

EPO simple patent family

WO 9902650 A1 19990121; EP 1002053 A1 20000524; EP 1009803 A1 20000621; GB 9721396 D0 19971210; WO 9902649 A1 19990121

INPADOC legal status


2002-09-11 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20020305

2000-05-24 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 19990522

2000-05-24 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): DE FR GB IT