Global patent index - EP 1002083 A1

EP 1002083 A1 2000-05-24 - APROTININ VARIANTS WITH IMPROVED PROPERTIES AND BIKUNINS OF APROTININ VARIANTS

Title (en)

APROTININ VARIANTS WITH IMPROVED PROPERTIES AND BIKUNINS OF APROTININ VARIANTS

Title (de)

APROTININVARIANTEN MIT VERBESSERTEN EIGENSCHAFTEN UND BIKUNINE VON APROTININVARIANTEN

Title (fr)

VARIANTES D'APROTININE A PROPRIETES AMELIOREES ET BIKUNINES DE VARIANTES D'APROTININE

Publication

EP 1002083 A1 (DE)

Application

EP 98930775 A

Priority

  • DE 19725014 A
  • EP 9803259 W

Abstract (en)

[origin: DE19725014A1] The invention relates to aprotinin variants with a net charge of +3 to -3 at pH 7. In the binding site, the amino acid radical 10 is Ser, the amino acid radical 13 is Ile, Phe or Leu, the amino acid radical 15 is Arg, the amino acid radical 17 is Tyr, Leu or Arg, and the amino acid radical 19 is Thr or Lys. The invention also relates to bikunins, comprising two aprotinin variants linked by a spacer with several amino acid radicals. One of said aprotinin variants is a) an aprotinin variant wherein the amino acid radical 13 is Ile, Phe or Leu, the amino acid radical 15 is Arg, Val or Leu, the amino acid radical 17 is Tyr, Leu or Ala, the amino acid radical 19 is Thr or Lys, the amino acid radical 39 is Arg or Leu, and the amino acid radical 46 is Leu or Lys; or b) an inventive aprotinin variant as defined above. The invention also relates to medicaments containing one or several of said aprotinin variants or bikunins, DNA sequences which give the coding for one of said aprotinin variants or bikunins, micro-organisms containing a DNA sequence of this type, and a method for producing said aprotinin variants and bikunins using micro-organisms.

IPC 1-7 (main, further and additional classification)

C12N 15/15; A61K 38/57; C07K 14/81; C12N 1/19; C12N 1/21

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); A61K 38/55 (2006.01); A61P 7/02 (2006.01); A61P 9/10 (2006.01); A61P 41/00 (2006.01); C07K 14/81 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 15/15 (2006.01); C12P 21/02 (2006.01); A61K 38/00 (2006.01)

CPC (invention and additional information)

C07K 14/8117 (2013.01); A61K 38/00 (2013.01); C07K 2319/00 (2013.01)

Citation (search report)

See references of WO 9856916A1

Designated contracting state (EPC)

DE ES FR GB IT

EPO simple patent family

DE 19725014 A1 19981217; AU 8108998 A 19981230; EP 1002083 A1 20000524; JP 2002503958 A 20020205; WO 9856916 A1 19981217

INPADOC legal status

2004-07-21 [18D] DEEMED TO BE WITHDRAWN

- Ref Legal Event Code: 18D

- Effective date: 20040101

2000-05-24 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 20000113

2000-05-24 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): DE ES FR GB IT