Global patent index - EP 1002101 A2

EP 1002101 A2 2000-05-24 - ANTIFREEZE PROTEINS, DNA AND EXPRESSION SYSTEMS

Title (en)

ANTIFREEZE PROTEINS, DNA AND EXPRESSION SYSTEMS

Title (de)

GEFRIERSCHUTZPROTEINE, DEREN DNS UND VEKTORSYSTEME

Title (fr)

PROTEINES ANTIGEL, ADN ET SYSTEMES D'EXPRESSION

Publication

EP 1002101 A2 (EN)

Application

EP 98937376 A

Priority

  • CA 9800745 W
  • US 90387297 A

Abstract (en)

[origin: WO9906565A2] &sect& he winter rye, upon cold-induction or aclimation, produces a family of antifreeze proteins that are similar to pathogen-related proteins. Two of these proteins, both of which are chitinase-like proteins, are cloned using molecular biology techniques and are expressed in bacterial and yeast (Pichia) systems and Arabidopsis thaliana. The recombinant proteins showed both chitinase and antifreeze activities. The invention includes the DNA and protein sequences of the chitinase-like antifreeze proteins, any modifications of the said sequences, the expression of these proteins and their appplication in agriculture, food industry and medicine.

[origin: WO9906565A2] &sect& he winter rye, upon cold-induction or aclimation, produces a family of antifreeze proteins that are similar to pathogen-related proteins. Two of these proteins, both of which are chitinase-like proteins, are cloned using molecular biology techniques and are expressed in bacterial and yeast (Pichia) systems and Arabidopsis thaliana. The recombinant proteins showed both chitinase and antifreeze activities. The invention includes the DNA and protein sequences of the chitinase-like antifreeze proteins, any modifications of the said sequences, the expression of these proteins and their appplication in agriculture, food industry and medicine.

IPC 1-7 (main, further and additional classification)

C12N 15/56; A01H 5/00; A01K 67/027; A23G 9/00; A23L 3/36; C12N 1/19; C12N 1/21; C12N 9/24; C12N 15/82

IPC 8 full level (invention and additional information)

C07K 14/415 (2006.01); C12N 1/21 (2006.01); C12N 9/24 (2006.01); C12N 15/29 (2006.01); C12N 15/82 (2006.01); A61K 38/00 (2006.01)

CPC (invention and additional information)

C07K 14/415 (2013.01); C12N 9/2442 (2013.01); C12N 15/8261 (2013.01); C12N 15/8273 (2013.01); C12Y 302/01014 (2013.01); A01K 2217/05 (2013.01); A61K 38/00 (2013.01); C07K 2319/02 (2013.01)

Citation (search report)

See references of WO 9906565A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9906565 A2 19990211; WO 9906565 A3 19990603; AU 8620698 A 19990222; CA 2299620 A1 19990211; CN 1273604 A 20001115; EP 1002101 A2 20000524

INPADOC legal status

2004-12-29 [18D] DEEMED TO BE WITHDRAWN

- Ref Legal Event Code: 18D

- Effective date: 20040203

2003-08-27 [17Q] FIRST EXAMINATION REPORT

- Ref Legal Event Code: 17Q

- Effective date: 20030709

2000-12-06 [RIN1] INVENTOR (CORRECTION)

- Ref Legal Event Code: RIN1

- Inventor name: HEW, CHOY

2000-12-06 [RIN1] INVENTOR (CORRECTION)

- Ref Legal Event Code: RIN1

- Inventor name: XIONG, FEI

2000-12-06 [RIN1] INVENTOR (CORRECTION)

- Ref Legal Event Code: RIN1

- Inventor name: MOFFATT, BARBARA

2000-12-06 [RIN1] INVENTOR (CORRECTION)

- Ref Legal Event Code: RIN1

- Inventor name: GRIFFITH, MARILYN

2000-05-24 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 20000229

2000-05-24 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE