Global Patent Index - EP 1002137 A1

EP 1002137 A1 2000-05-24 - DENATURING MULTI ION POLYNUCLEOTIDE CHROMATOGRAPHY FOR DETECTING MUTATIONS

Title (en)

DENATURING MULTI ION POLYNUCLEOTIDE CHROMATOGRAPHY FOR DETECTING MUTATIONS

Title (de)

DENATURIERENDE MULTIIONEN-POLYNUKLEOTIDCHROMATOGRAPHIE ZUR ERKENNUNG VON MUTATIONEN

Title (fr)

CHROMATOGRAPHIE PAR DENATURATION D'IONS APPARIES DE POLYNUCLEOTIDE PERMETTANT DE DETECTER DES MUTATIONS

Publication

EP 1002137 A1 (EN)

Application

EP 98939218 A

Priority

  • US 9816260 W
  • US 5478897 P
  • US 5601297 P
  • US 5650097 P
  • US 6144597 P
  • US 6269097 P
  • US 6726997 P
  • US 7057298 P
  • US 7058598 P
  • US 9384498 P
  • US 12910598 A

Abstract (en)

[origin: WO9907899A1] The present invention is directed to improved methods for detection of mutations in DNA using Denaturing Matched Ion Polynucleotide Chromatography (DMIPC). The invention includes the following aspects: analysis of PCR amplification products to identify factors that affect PCR replication fidelity; design of PCR primers; selection of an optimal temperature for performing DMIPC; selection of the mobile phase composition for gradient elution; methods for column preparation and maintenance; and methods for preparing polynucleotide samples prior to chromatographic analysis.

IPC 1-7 (main, further and additional classification)

C12Q 1/70; C07H 21/00; C07H 21/02; C12M 1/40; C12P 19/34; C12Q 1/68

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); C07H 1/06 (2006.01); C07H 21/04 (2006.01); C12M 1/36 (2006.01); C12N 15/00 (2006.01); C12N 15/01 (2006.01); C12N 15/10 (2006.01); C12P 19/34 (2006.01); C12Q 1/68 (2006.01); C12Q 1/70 (2006.01); G01N 30/88 (2006.01)

CPC (invention and additional information)

C12Q 1/6834 (2013.01); C12N 15/101 (2013.01); C12Q 1/6827 (2013.01)

Combination set (CPC)

C12Q 1/6827 + C12Q 2565/137 + C12Q 2527/107

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family

WO 9907899 A1 19990218; AU 750394 B2 20020718; AU 8769398 A 19990301; CA 2298019 A1 19990218; EP 1002137 A1 20000524; EP 1002137 A4 20030102; JP 2001512702 A 20010828