Global Patent Index - EP 1003759 A2

EP 1003759 A2 2000-05-31 - MICROSTRUCTURES FOR THE MANIPULATION OF FLUID SAMPLES

Title (en)

MICROSTRUCTURES FOR THE MANIPULATION OF FLUID SAMPLES

Title (de)

MIKROSTRUKTUREN FÜR DIE MANIPULATION VON FLÜSSIGEN PROBEN

Title (fr)

MICROSTRUCTURES PERMETTANT DE MANIPULER DES ECHANTILLONS FLUIDES

Publication

EP 1003759 A2 (EN)

Application

EP 98940889 A

Priority

  • US 9816870 W
  • US 91043497 A
  • US 11545498 A

Abstract (en)

[origin: WO9909042A2] The invention presents a microfluidic device and method for separating a desired material, such as nucleic acid, from other materials in a fluid sample. In a preferred embodiment, the device comprises a microfabricated chip (20) having an inlet port (28), an outlet port (30), and an extraction chamber (26) in fluid communication with the ports. The chamber (26) has internal attachment surfaces for capturing the desired material from the fluid sample as the sample flows continuously through the chamber. The captured material may then be eluted by forcing an elution fluid to flow through the chamber (26), thus releasing the material from the internal surfaces into the elution fluid. The flow-through design of the device allows target material from a relatively large volume of fluid sample to be concentrated into a much smaller volume of elution fluid. The internal surfaces are preferably formed by an array of columns (32) integrally formed with a wall of the chamber (26) and extending into the chamber (26). The collumns (32) provide a large surface area for capturing the desired material. The device also preferably includes an integrated heater (34) for increasing elution efficiency.

IPC 1-7 (main, further and additional classification)

C07H 1/00

IPC 8 full level (invention and additional information)

G01N 31/20 (2006.01); B01D 15/08 (2006.01); B01F 5/04 (2006.01); B01F 5/06 (2006.01); B01F 13/00 (2006.01); B01J 19/00 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); C07H 1/00 (2006.01); C07H 21/00 (2006.01); G01N 27/447 (2006.01); G01N 33/53 (2006.01); G01N 37/00 (2006.01); G01N 1/34 (2006.01)

CPC (invention and additional information)

B01L 3/502753 (2013.01); B01F 5/0403 (2013.01); B01F 5/0645 (2013.01); B01F 13/0059 (2013.01); B01J 19/0093 (2013.01); B01L 3/502707 (2013.01); C07H 1/00 (2013.01); B01L 2200/0631 (2013.01); B01L 2200/0636 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0883 (2013.01); B01L 2300/1827 (2013.01); B01L 2400/0421 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/086 (2013.01); G01N 1/405 (2013.01)

Citation (search report)

See references of WO 9909042A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9909042 A2 19990225; WO 9909042 A3 19990506; AU 745989 B2 20020411; AU 8906698 A 19990308; CA 2301309 A1 19990225; EP 1003759 A2 20000531; JP 2001515216 A 20010918

INPADOC legal status


2010-08-25 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20100302

2001-01-24 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20001207

2000-05-31 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000202

2000-05-31 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE