EP 1003759 A2 20000531 - MICROSTRUCTURES FOR THE MANIPULATION OF FLUID SAMPLES
Title (en)
MICROSTRUCTURES FOR THE MANIPULATION OF FLUID SAMPLES
Title (de)
MIKROSTRUKTUREN FÜR DIE MANIPULATION VON FLÜSSIGEN PROBEN
Title (fr)
MICROSTRUCTURES PERMETTANT DE MANIPULER DES ECHANTILLONS FLUIDES
Publication
Application
Priority
- US 9816870 W 19980813
- US 91043497 A 19970813
- US 11545498 A 19980714
Abstract (en)
[origin: WO9909042A2] The invention presents a microfluidic device and method for separating a desired material, such as nucleic acid, from other materials in a fluid sample. In a preferred embodiment, the device comprises a microfabricated chip (20) having an inlet port (28), an outlet port (30), and an extraction chamber (26) in fluid communication with the ports. The chamber (26) has internal attachment surfaces for capturing the desired material from the fluid sample as the sample flows continuously through the chamber. The captured material may then be eluted by forcing an elution fluid to flow through the chamber (26), thus releasing the material from the internal surfaces into the elution fluid. The flow-through design of the device allows target material from a relatively large volume of fluid sample to be concentrated into a much smaller volume of elution fluid. The internal surfaces are preferably formed by an array of columns (32) integrally formed with a wall of the chamber (26) and extending into the chamber (26). The collumns (32) provide a large surface area for capturing the desired material. The device also preferably includes an integrated heater (34) for increasing elution efficiency.
IPC 1-7
IPC 8 full level
B01D 15/08 (2006.01); B01F 5/04 (2006.01); B01F 5/06 (2006.01); B01F 13/00 (2006.01); B01J 19/00 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); C07H 1/00 (2006.01); C07H 21/00 (2006.01); G01N 27/447 (2006.01); G01N 31/20 (2006.01); G01N 33/53 (2006.01); G01N 37/00 (2006.01); G01N 1/34 (2006.01)
CPC (source: EP)
B01F 25/31 (2022.01); B01F 25/43231 (2022.01); B01F 33/30 (2022.01); B01J 19/0093 (2013.01); B01L 3/502707 (2013.01); B01L 3/502753 (2013.01); C07H 1/00 (2013.01); B01L 2200/0631 (2013.01); B01L 2200/0636 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0883 (2013.01); B01L 2300/1827 (2013.01); B01L 2400/0421 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/086 (2013.01); G01N 1/405 (2013.01)
Citation (search report)
See references of WO 9909042A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9909042 A2 19990225; WO 9909042 A3 19990506; AU 745989 B2 20020411; AU 8906698 A 19990308; CA 2301309 A1 19990225; EP 1003759 A2 20000531; JP 2001515216 A 20010918
DOCDB simple family (application)
US 9816870 W 19980813; AU 8906698 A 19980813; CA 2301309 A 19980813; EP 98940889 A 19980813; JP 2000509721 A 19980813