Title (en)

METHOD FOR ENZYMATICALLY PRODUCING GUANOSINE DIPHOSPHATE-6-DESOXYHEXOSES AND THE USE THEREOF FOR PRODUCING OLIGOSACCHARIDES

Title (de)

VERFAHREN ZUR ENZYMATISCHEN HERSTELLUNG VON GUANOSINDIPHOSPHAT-6-DESOXYHEXOSEN UND DEREN VERWENDUNG ZUR HERSTELLUNG VON OLIGOSACCHARIDEN

Title (fr)

PROCEDE DE PRODUCTION ENZYMATIQUE DE GUANOSINDIPHOSPHAT-6-DESOXYHEXOSES ET LEUR UTILISATION POUR LA PRODUCTION D'OLIGOSACCHARIDES

Publication

EP 1005554 A2 20000607 (DE)

Application

EP 98943894 A 19980818

Priority

• DE 19735994 A 19970819
• EP 9805242 W 19980818

Abstract (en)

[origin: DE19735994A1] Enzymatic synthesis of guanosinediphosphate (GDP)-6-deoxyhexoses (I) from simple nutrients or GDP-D-mannose (II). Independent claims are also included for the following: (a) isolation and cloning of the genes manB (phosphomannomutase), manC (GDP-D-mannose synthase), gmd (GDP-D-mannose-4,6-dehydratase), rfbD (as gmd), wcaG (GDP-4-keto-6-deoxymannose-3,5-epimerase-4-reductase) or rffT (fucosyl transferase) from Escherichia coli, wbcH (fucosyl transferase) from Yersinia enterocolitica, fucT (fucosyl transferase) from Helicobacter pylori, the gene for perosaminyl tranferase from Vibrio cholerae O1 or other bacteria, in suitable cloning vectors and transformation into bacteria or fungi; (b) active expression of these genes; (c) transfer of GDP-activated sugars using glycosyl transferases.

IPC 1-7

C12N 15/52; C12P 19/32; C12P 19/18

IPC 8 full level

C12N 15/09 (2006.01); C12P 19/18 (2006.01); C12P 19/32 (2006.01)

CPC (source: EP)

C12P 19/18 (2013.01); C12P 19/32 (2013.01)

Citation (search report)

See references of WO 9909180A2

Designated contracting state (EPC)

AT BE CH DE DK ES FR GB IT LI NL SE

DOCDB simple family (publication)

DE 19735994 A1 19990225; EP 1005554 A2 20000607; JP 2001514896 A 20010918; WO 9909180 A2 19990225; WO 9909180 A3 19990415

DOCDB simple family (application)

DE 19735994 A 19970819; EP 9805242 W 19980818; EP 98943894 A 19980818; JP 2000509844 A 19980818