Global Patent Index - EP 1005568 A1

EP 1005568 A1 2000-06-07 - PROCESS TO DETECT TOXIC SUBSTANCES IN THE ENVIRONMENT

Title (en)

PROCESS TO DETECT TOXIC SUBSTANCES IN THE ENVIRONMENT

Title (de)

METHODE ZUR BESTIMMUNG TOXISCHER SUBSTANZEN

Title (fr)

PROCEDE SERVANT A DETECTER DES SUBSTANCES TOXIQUES DANS L'ENVIRONNEMENT

Publication

EP 1005568 A1 (EN)

Application

EP 98945188 A

Priority

  • EP 9805043 W
  • IT TO970751 A

Abstract (en)

[origin: WO9909202A1] The process according to the invention includes the stages consisting in: placing in an environment to be biomonitored, like air, water, ground, food, clothes, etc., a biosensor consisting in wild type or genetically engineered Dictyostelium cells in conditions apt to development; leaving the cells for 20 hr in the same environment; evaluate the change in morphology of the cell aggregates or the expression of a reporter gene during development. The biosensor for carrying out the process consists of Dictyostelium cells of wild type or transformed with a plasmid containing the gene for a reporter protein. The gene is fused to control elements specific for different Dictyostelium genes, so that the reporter gene is expressed and the reporter protein is synthesised in strains transformed with different plasmids at different times of development, only when the specific regulatory sequences each plasmid contains are activated. The presence of the reporter protein can be detected by naked eye.

IPC 1-7 (main, further and additional classification)

C12Q 1/00; C12Q 1/02

IPC 8 full level (invention and additional information)

C12Q 1/02 (2006.01)

CPC (invention and additional information)

C12Q 1/025 (2013.01); G01N 2520/00 (2013.01)

Citation (search report)

See references of WO 9909202A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9909202 A1 19990225; AU 9259298 A 19990308; EP 1005568 A1 20000607; IT TO970751 A1 19990222

INPADOC legal status


2001-09-05 [18D] DEEMED TO BE WITHDRAWN

- Ref Legal Event Code: 18D

- Effective date: 20010301

2000-06-07 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 20000320

2000-06-07 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE