EP 1005653 A1 20000607 - METHOD OF PRODUCING A SUBTRACTION LIBRARY
Title (en)
METHOD OF PRODUCING A SUBTRACTION LIBRARY
Title (de)
VERFAHREN ZUR HERSTELLUNG EINER SUBTRAKTIONS-BIBLIOTHEK
Title (fr)
PROCEDE DE REALISATION D'ECHANTILLOTHEQUE SOUSTRACTIVE
Publication
Application
Priority
- US 9805644 W 19980324
- US 4168897 P 19970324
Abstract (en)
[origin: WO9843088A1] A method of producing a subtraction library using a collection of defined sequences is described. The method involves providing a surface containing a collection consisting of known nucleic acid sequences, which is subsequently contacted with a library containing undefined sequences under appropriate hybridization conditions. Any non-hybridized DNA is recovered and sequenced. The resulting sequenced, non-hybridizing DNA forms a subtraction library which contains sequences which were present in the library, and which differ from the sequences of the collection. Also described is a subtraction library prepared according to the method of the invention. Methods are also provided for making and using subtracted probes.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6809 (2018.01); C12Q 1/6837 (2018.01); G01N 33/15 (2006.01); G01N 33/50 (2006.01); G01N 33/53 (2006.01); G01N 33/566 (2006.01)
CPC (source: EP)
C12Q 1/6809 (2013.01); C12Q 1/6837 (2013.01)
Designated contracting state (EPC)
BE CH DE ES FR GB IT LI NL
DOCDB simple family (publication)
WO 9843088 A1 19981001; CA 2284481 A1 19981001; EP 1005653 A1 20000607; EP 1005653 A4 20040506; JP 2002507275 A 20020305
DOCDB simple family (application)
US 9805644 W 19980324; CA 2284481 A 19980324; EP 98913043 A 19980324; JP 54586898 A 19980324