Global Patent Index - EP 1005653 A1

EP 1005653 A1 2000-06-07 - METHOD OF PRODUCING A SUBTRACTION LIBRARY

Title (en)

METHOD OF PRODUCING A SUBTRACTION LIBRARY

Title (de)

VERFAHREN ZUR HERSTELLUNG EINER SUBTRAKTIONS-BIBLIOTHEK

Title (fr)

PROCEDE DE REALISATION D'ECHANTILLOTHEQUE SOUSTRACTIVE

Publication

EP 1005653 A1 (EN)

Application

EP 98913043 A

Priority

  • US 9805644 W
  • US 4168897 P

Abstract (en)

[origin: WO9843088A1] A method of producing a subtraction library using a collection of defined sequences is described. The method involves providing a surface containing a collection consisting of known nucleic acid sequences, which is subsequently contacted with a library containing undefined sequences under appropriate hybridization conditions. Any non-hybridized DNA is recovered and sequenced. The resulting sequenced, non-hybridizing DNA forms a subtraction library which contains sequences which were present in the library, and which differ from the sequences of the collection. Also described is a subtraction library prepared according to the method of the invention. Methods are also provided for making and using subtracted probes.

IPC 1-7 (main, further and additional classification)

G01N 33/566; C12N 15/10; C12Q 1/68

IPC 8 full level (invention and additional information)

G01N 33/53 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); G01N 33/15 (2006.01); G01N 33/50 (2006.01); G01N 33/566 (2006.01)

CPC (invention and additional information)

C12Q 1/6809 (2013.01); C12Q 1/6837 (2013.01)

Designated contracting state (EPC)

BE CH DE ES FR GB IT LI NL

EPO simple patent family

WO 9843088 A1 19981001; CA 2284481 A1 19981001; EP 1005653 A1 20000607; EP 1005653 A4 20040506; JP 2002507275 A 20020305

INPADOC legal status


2004-12-29 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20040504

2004-05-06 [A4] SUPPLEMENTARY SEARCH REPORT

- Effective date: 20040318

2004-05-06 [RIC1] CLASSIFICATION (CORRECTION)

- IPC: 7G 01N 33/566 A

2004-05-06 [RIC1] CLASSIFICATION (CORRECTION)

- IPC: 7C 12Q 1/68 B

2004-05-06 [RIC1] CLASSIFICATION (CORRECTION)

- IPC: 7C 12N 15/10 B

2000-06-07 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 19991001

2000-06-07 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): BE CH DE ES FR GB IT LI NL