Global Patent Index - EP 1005653 A4

EP 1005653 A4 20040506 - METHOD OF PRODUCING A SUBTRACTION LIBRARY

Title (en)

METHOD OF PRODUCING A SUBTRACTION LIBRARY

Title (de)

VERFAHREN ZUR HERSTELLUNG EINER SUBTRAKTIONS-BIBLIOTHEK

Title (fr)

PROCEDE DE REALISATION D'ECHANTILLOTHEQUE SOUSTRACTIVE

Publication

EP 1005653 A4 20040506 (EN)

Application

EP 98913043 A 19980324

Priority

  • US 9805644 W 19980324
  • US 4168897 P 19970324

Abstract (en)

[origin: WO9843088A1] A method of producing a subtraction library using a collection of defined sequences is described. The method involves providing a surface containing a collection consisting of known nucleic acid sequences, which is subsequently contacted with a library containing undefined sequences under appropriate hybridization conditions. Any non-hybridized DNA is recovered and sequenced. The resulting sequenced, non-hybridizing DNA forms a subtraction library which contains sequences which were present in the library, and which differ from the sequences of the collection. Also described is a subtraction library prepared according to the method of the invention. Methods are also provided for making and using subtracted probes.

IPC 1-7

G01N 33/566; C12Q 1/68; C12N 15/10

IPC 8 full level

C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6809 (2018.01); C12Q 1/6837 (2018.01); G01N 33/15 (2006.01); G01N 33/50 (2006.01); G01N 33/53 (2006.01); G01N 33/566 (2006.01)

CPC (source: EP)

C12Q 1/6809 (2013.01); C12Q 1/6837 (2013.01)

Citation (search report)

  • [X] WO 9318186 A1 19930916 - UNIV CALIFORNIA [US]
  • [X] EP 0372524 A2 19900613 - GEN HOSPITAL CORP [US]
  • [X] EP 0533952 A1 19930331 - SUMITOMO ELECTRIC INDUSTRIES [JP]
  • [X] ROBERTSON N G ET AL: "ISOLATION OF NOVEL AND KNOWN GENES FROM A HUMAN FETAL COCHLEAR CDNA LIBRARY USING SUBSTRACTIVE HYBRIDIZATION AND DIFFERENTIAL SCREENING", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 23, no. 1, 1 September 1994 (1994-09-01), pages 42 - 50, XP001012412, ISSN: 0888-7543
  • [X] LOPEZ-FERNANDEZ L A ET AL: "CONSTRUCTION OF SUBTRACTIVE CDNA LIBRARIES FROM LIMITED AMOUNTS OF MRNA AND MULTIPLE CYCLES OF SUBTRACTION", BIOTECHNIQUES, EATON PUBLISHING, NATICK, US, vol. 15, no. 4, 1 October 1993 (1993-10-01), pages 654,656,658 - 659, XP000402907, ISSN: 0736-6205
  • [X] HARA E ET AL: "Subtractive cDNA cloning using oligo(dT)30-latex and PCR: isolation of cDNA clones specific to undifferentiated human embryonal carcinoma cells", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 19, no. 25, December 1991 (1991-12-01), pages 7097 - 7104, XP002209142, ISSN: 0305-1048
  • See references of WO 9843088A1

Designated contracting state (EPC)

BE CH DE ES FR GB IT LI NL

DOCDB simple family (publication)

WO 9843088 A1 19981001; CA 2284481 A1 19981001; EP 1005653 A1 20000607; EP 1005653 A4 20040506; JP 2002507275 A 20020305

DOCDB simple family (application)

US 9805644 W 19980324; CA 2284481 A 19980324; EP 98913043 A 19980324; JP 54586898 A 19980324