EP 1007722 A1 20000614 - COLD-ACTIVE PROTEASE CP-58 AND PSYCHROTROPHIC BACTERIA
Title (en)
COLD-ACTIVE PROTEASE CP-58 AND PSYCHROTROPHIC BACTERIA
Title (de)
KÄLTEAKTIVE PROTEASE CP-58 UND PSYCHROTROPHISCHE BAKTERIEN
Title (fr)
PROTEASE ACTIVE A FROID CP-58 ET BACTERIES PSYCHROTROPHES
Publication
Application
Priority
- JP 2961696 A 19960216
- US 9702437 W 19970214
Abstract (en)
[origin: WO9730172A1] A cold-active protease is here disclosed which has the following physicochemical properties: (a) specific activity and substrate specificity: the protease acts on casein, gelatin, albumin and hemoglobin to specifically decompose them in the order of casein, gelatin, albumin and hemoglobin; (b) optimal pH: 7.5 to 8.0; (c) pH stability: the protease is stable at a pH in the range of 5.5 to 10.5 at 20 DEG C for 1 hour; (d) optimal temperature: 20 DEG C at pH 10.5 and 40 DEG C at pH 8.0; (e) temperature stability: at pH 10.5 for 1 hour, the protease is scarcely inactivated at a temperature of 10 DEG C to 30 DEG C, but it is inactivated at 40 DEG C as much as about 30 % and completely inactivated at 50 DEG C; (f) enzyme activity: the protease has about 60 % or more of its maximum activity at 20 DEG C; (g) the active center of the enzyme is a metallic ion; and (h) the molecular weight of the protease is about 58 kDa as measured by SDS-PAGE.
IPC 1-7
IPC 8 full level
C11D 3/386 (2006.01); C12N 1/20 (2006.01); C12N 9/52 (2006.01); C12P 21/04 (2006.01); C12R 1/43 (2006.01)
CPC (source: EP)
C11D 3/386 (2013.01); C12N 9/52 (2013.01); C12Y 304/00 (2013.01)
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE
DOCDB simple family (publication)
WO 9730172 A1 19970821; AR 005831 A1 19990714; EP 1007722 A1 20000614; EP 1007722 A4 20021204; JP H09224666 A 19970902
DOCDB simple family (application)
US 9702437 W 19970214; AR P970100599 A 19970214; EP 97905991 A 19970214; JP 2961696 A 19960216