Global patent index - EP 1007722 A1

EP 1007722 A1 2000-06-14 - COLD-ACTIVE PROTEASE CP-58 AND PSYCHROTROPHIC BACTERIA

Title (en)

COLD-ACTIVE PROTEASE CP-58 AND PSYCHROTROPHIC BACTERIA

Title (de)

KÄLTEAKTIVE PROTEASE CP-58 UND PSYCHROTROPHISCHE BAKTERIEN

Title (fr)

PROTEASE ACTIVE A FROID CP-58 ET BACTERIES PSYCHROTROPHES

Publication

EP 1007722 A1 (EN)

Application

EP 97905991 A

Priority

  • JP 2961696 A
  • US 9702437 W

Abstract (en)

[origin: WO9730172A1] A cold-active protease is here disclosed which has the following physicochemical properties: (a) specific activity and substrate specificity: the protease acts on casein, gelatin, albumin and hemoglobin to specifically decompose them in the order of casein, gelatin, albumin and hemoglobin; (b) optimal pH: 7.5 to 8.0; (c) pH stability: the protease is stable at a pH in the range of 5.5 to 10.5 at 20 DEG C for 1 hour; (d) optimal temperature: 20 DEG C at pH 10.5 and 40 DEG C at pH 8.0; (e) temperature stability: at pH 10.5 for 1 hour, the protease is scarcely inactivated at a temperature of 10 DEG C to 30 DEG C, but it is inactivated at 40 DEG C as much as about 30 % and completely inactivated at 50 DEG C; (f) enzyme activity: the protease has about 60 % or more of its maximum activity at 20 DEG C; (g) the active center of the enzyme is a metallic ion; and (h) the molecular weight of the protease is about 58 kDa as measured by SDS-PAGE.

IPC 1-7 (main, further and additional classification)

C12P 21/04; A23J 1/00; A61K 38/00; C12N 9/52

IPC 8 full level (invention and additional information)

C11D 3/386 (2006.01); C12N 1/20 (2006.01); C12N 9/52 (2006.01); C12P 21/04 (2006.01); C12R 1/43 (2006.01)

CPC (invention and additional information)

C12Y 304/00 (2013.01); C11D 3/386 (2013.01); C12N 9/52 (2013.01)

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE

EPO simple patent family

WO 9730172 A1 19970821; AR 005831 A1 19990714; EP 1007722 A1 20000614; EP 1007722 A4 20021204; JP H09224666 A 19970902

INPADOC legal status

2003-07-16 [18D] DEEMED TO BE WITHDRAWN

- Ref Legal Event Code: 18D

- Effective date: 20030108

2002-12-04 [A4] SUPPLEMENTARY SEARCH REPORT

- Ref Legal Event Code: A4

- Effective date: 20021017

2002-12-04 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE

2002-11-20 [RIC1] CLASSIFICATION (CORRECTION)

- Ref Legal Event Code: RIC1

- Free Format Text: 7C 12P 21/04 A, 7C 12N 9/52 B, 7A 61K 38/00 B, 7C 11D 3/386 B

2000-06-14 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 19980902

2000-06-14 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE