EP 1007722 A4 20021204 - COLD-ACTIVE PROTEASE CP-58 AND PSYCHROTROPHIC BACTERIA
Title (en)
COLD-ACTIVE PROTEASE CP-58 AND PSYCHROTROPHIC BACTERIA
Title (de)
KÄLTEAKTIVE PROTEASE CP-58 UND PSYCHROTROPHISCHE BAKTERIEN
Title (fr)
PROTEASE ACTIVE A FROID CP-58 ET BACTERIES PSYCHROTROPHES
Publication
Application
Priority
- JP 2961696 A 19960216
- US 9702437 W 19970214
Abstract (en)
[origin: WO9730172A1] A cold-active protease is here disclosed which has the following physicochemical properties: (a) specific activity and substrate specificity: the protease acts on casein, gelatin, albumin and hemoglobin to specifically decompose them in the order of casein, gelatin, albumin and hemoglobin; (b) optimal pH: 7.5 to 8.0; (c) pH stability: the protease is stable at a pH in the range of 5.5 to 10.5 at 20 DEG C for 1 hour; (d) optimal temperature: 20 DEG C at pH 10.5 and 40 DEG C at pH 8.0; (e) temperature stability: at pH 10.5 for 1 hour, the protease is scarcely inactivated at a temperature of 10 DEG C to 30 DEG C, but it is inactivated at 40 DEG C as much as about 30 % and completely inactivated at 50 DEG C; (f) enzyme activity: the protease has about 60 % or more of its maximum activity at 20 DEG C; (g) the active center of the enzyme is a metallic ion; and (h) the molecular weight of the protease is about 58 kDa as measured by SDS-PAGE.
IPC 1-7
IPC 8 full level
C11D 3/386 (2006.01); C12N 1/20 (2006.01); C12N 9/52 (2006.01); C12P 21/04 (2006.01); C12R 1/43 (2006.01)
CPC (source: EP)
C11D 3/386 (2013.01); C12N 9/52 (2013.01); C12Y 304/00 (2013.01)
Citation (search report)
- [PXA] WO 9727313 A1 19970731 - PROCTER & GAMBLE [US], et al
- [XA] NAKAJIMA M ET AL: "PURIFICATION AND PROPERTIES OF AN EXTRACELLULAR PROTEINASE OF PSYCHROPHILIC ESCHERICHIA FREUNDII", EUROPEAN JOURNAL OF BIOCHEMISTRY, BERLIN, DE, vol. 44, 1974, pages 87 - 96, XP002913393, ISSN: 0014-2956
- [XA] DAVAIL STEPHANE ET AL: "Cold adaptation of proteins: Purification, characterization, and sequence of the heat-labile subtilisin from the antarctic psychrophile Bacillus TA41.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 269, no. 26, 1994, pages 17448 - 17453, XP002214975, ISSN: 0021-9258
- [XA] PATENT ABSTRACTS OF JAPAN vol. 011, no. 141 (C - 421) 8 May 1987 (1987-05-08)
- [T] MORITA YASUTAKA ET AL: "Purification and characterization of a cold-active protease from psychrotrophic Serratia marcescens AP3801", JAOCS J AM OIL CHEM SOC;JAOCS, JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY NOV 1997 AMERICAN OIL CHEMISTS' SOC, CHAMPAIGN, IL, USA, vol. 74, no. 11, November 1997 (1997-11-01), pages 1377 - 1383, XP001093780
- See references of WO 9730172A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE
DOCDB simple family (publication)
WO 9730172 A1 19970821; AR 005831 A1 19990714; EP 1007722 A1 20000614; EP 1007722 A4 20021204; JP H09224666 A 19970902
DOCDB simple family (application)
US 9702437 W 19970214; AR P970100599 A 19970214; EP 97905991 A 19970214; JP 2961696 A 19960216