Global Patent Index - EP 1009862 A1

EP 1009862 A1 2000-06-21 - RAPID DETECTION AND IDENTIFICATION OF MICRO-ORGANISMS

Title (en)

RAPID DETECTION AND IDENTIFICATION OF MICRO-ORGANISMS

Title (de)

SCHNELLE DETECTION UBD IDENTIFIZIERUNG VON MIKROORGANISMEN

Title (fr)

DETECTION ET IDENTIFICATION RAPIDES DE MICRO-ORGANISMES

Publication

EP 1009862 A1 (EN)

Application

EP 98940684 A

Priority

  • EP 98940684 A
  • EP 97202618 A
  • NL 9800481 W

Abstract (en)

[origin: WO9910533A1] The invention relates to the field microbiology, more specifically to the field of detection, identification and quantification or enumeration of micro-organisms. Micro-organisms, such as viruses, plasmids, bacteria, yeasts, fungi, algae, protozoa, plant or animal cells, and other prokaryotic or eukaryotic cells are in general unicellular organisms with dimensions beneath the limits of vision which thus escape easy detection. The invention provides methods and means for use in situ staining of micro-organisms comprising: a) mixing a material containing at least one micro-organism with a composition which can (partly) degrade a cell wall or cell membrane of a micro-organism thereby allowing for penetration through said wall and/or membrane of a (labelled) probe into said micro-organism, b) fixing said micro-organism with a fixative to retain its individual corpuscular character, c) reacting said probe with an antigen or nucleic acid molecule present in said micro-organism and d) detecting the presence of said probe in said micro-organism.

IPC 1-7 (main, further and additional classification)

C12Q 1/68; C07H 21/00; G01N 33/569

IPC 8 full level (invention and additional information)

C07H 21/00 (2006.01); C12Q 1/68 (2006.01); G01N 33/569 (2006.01)

CPC (invention and additional information)

C12Q 1/6841 (2013.01)

Citation (search report)

See references of WO 9910533A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9910533 A1 19990304; AU 8890498 A 19990316; EP 1009862 A1 20000621

INPADOC legal status


2003-09-03 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20030301

2000-06-21 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000327

2000-06-21 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE