EP 1009862 A1 20000621 - RAPID DETECTION AND IDENTIFICATION OF MICRO-ORGANISMS
Title (en)
RAPID DETECTION AND IDENTIFICATION OF MICRO-ORGANISMS
Title (de)
SCHNELLE DETECTION UBD IDENTIFIZIERUNG VON MIKROORGANISMEN
Title (fr)
DETECTION ET IDENTIFICATION RAPIDES DE MICRO-ORGANISMES
Publication
Application
Priority
- EP 98940684 A 19980826
- EP 97202618 A 19970826
- NL 9800481 W 19980826
Abstract (en)
[origin: WO9910533A1] The invention relates to the field microbiology, more specifically to the field of detection, identification and quantification or enumeration of micro-organisms. Micro-organisms, such as viruses, plasmids, bacteria, yeasts, fungi, algae, protozoa, plant or animal cells, and other prokaryotic or eukaryotic cells are in general unicellular organisms with dimensions beneath the limits of vision which thus escape easy detection. The invention provides methods and means for use in situ staining of micro-organisms comprising: a) mixing a material containing at least one micro-organism with a composition which can (partly) degrade a cell wall or cell membrane of a micro-organism thereby allowing for penetration through said wall and/or membrane of a (labelled) probe into said micro-organism, b) fixing said micro-organism with a fixative to retain its individual corpuscular character, c) reacting said probe with an antigen or nucleic acid molecule present in said micro-organism and d) detecting the presence of said probe in said micro-organism.
IPC 1-7
IPC 8 full level
C07H 21/00 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6841 (2018.01); G01N 33/569 (2006.01)
CPC (source: EP)
C12Q 1/6841 (2013.01)
Citation (search report)
See references of WO 9910533A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9910533 A1 19990304; AU 8890498 A 19990316; EP 1009862 A1 20000621
DOCDB simple family (application)
NL 9800481 W 19980826; AU 8890498 A 19980826; EP 98940684 A 19980826