Global Patent Index - EP 1012315 A1

EP 1012315 A1 20000628 - RECOMBINANT VECTORS

Title (en)

RECOMBINANT VECTORS

Title (de)

REKOMBINANTE VEKTOREN

Title (fr)

VECTEURS DE RECOMBINAISON

Publication

EP 1012315 A1 20000628 (EN)

Application

EP 97922279 A 19970507

Priority

  • SE 9700763 W 19970507
  • SE 9602803 A 19960718
  • SE 9603282 A 19960910

Abstract (en)

[origin: WO9803666A1] The present invention relates to recombinant vectors which are useful for direct selection of colonies harboring recombinant plasmids, on basis of the loss of expression of a gene coding for an AsiA polypeptide from E. coli bacteriophage T4, the expression of which would normally have resulted in toxicity to the bacterial host. The invention also relates to host cells harboring the said vectors, as well as to methods utilizing the said vectors, for the selection of nucleic acid clones.

IPC 1-7

C12N 15/70; C12N 15/74

IPC 8 full level

C07K 14/01 (2006.01); C07K 14/255 (2006.01); C12N 1/21 (2006.01); C12N 15/09 (2006.01); C12N 15/74 (2006.01); C12R 1/19 (2006.01)

CPC (source: EP)

C07K 14/005 (2013.01); C07K 14/255 (2013.01); C12N 15/74 (2013.01); C07K 2319/00 (2013.01); C12N 2795/10322 (2013.01)

Citation (search report)

See references of WO 9803666A1

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 9803666 A1 19980129; AU 2799297 A 19980210; EP 1012315 A1 20000628; JP 2000514658 A 20001107

DOCDB simple family (application)

SE 9700763 W 19970507; AU 2799297 A 19970507; EP 97922279 A 19970507; JP 50685098 A 19970507