EP 1015116 A1 20000705 - METHOD AND APPARATUS FOR THERMAL CYCLING AND FOR AUTOMATED SAMPLE PREPARATION WITH THERMAL CYCLING
Title (en)
METHOD AND APPARATUS FOR THERMAL CYCLING AND FOR AUTOMATED SAMPLE PREPARATION WITH THERMAL CYCLING
Title (de)
VERFAHREN UND VORRICHTUNG ZUR DURCHFÜHRUNG VON TEMPERATURZYKLEN UND ZUR AUTOMATISCHEN PROBENVORBEREITUNG MITTELS TEMPERATURZYKLEN
Title (fr)
PROCEDE ET DISPOSITIF DESTINES A LA PRODUCTION DE CYCLES THERMIQUES ET A L'AUTOMATISATION DE LA PREPARATION D'ECHANTILLONS AU MOYEN DE CES CYCLES THERMIQUES
Publication
Application
Priority
- CA 9700302 W 19970501
- US 64067296 A 19960501
- US 68449896 A 19960719
- US 70373096 A 19960827
Abstract (en)
[origin: WO9740939A1] An apparatus and method for thermally cycling a reaction mixture in a reaction vessel to expose the mixture to the varying temperatures necessary to, for example, achieve PCR amplification or the preparation of sequencing fragments using a cycle sequencing operation makes use of flow-through reaction vessels, such as capillary tubes, for the preparation and thermal cycling of reaction mixtures. In order to prevent loss of the reaction mixture from the vessels during heating, the thermal cycling apparatus of the invention provides means for reversibly sealing the proximal and distal ends of each reaction vessel. The proximal ends can be sealed by coupling to a pump which permits movement of the samples within the reaction vessels. As to the distal ends, the reaction vessels can be sealed by pressing the distal end of each vessel against a sealing element with a conformable surface, or by immersing the distal end of each vessel in the reservoir of liquid, preferably of an oil, that is not miscible with the reaction mixture.
IPC 1-7
IPC 8 full level
B01L 7/00 (2006.01); C12Q 1/68 (2006.01); C12Q 1/70 (2006.01); G01N 35/00 (2006.01)
CPC (source: EP)
B01L 7/52 (2013.01); B01L 7/525 (2013.01); G01N 2035/00237 (2013.01)
Citation (search report)
See references of WO 9740939A1
Designated contracting state (EPC)
DE FR GB
DOCDB simple family (publication)
WO 9740939 A1 19971106; AU 2378097 A 19971119; CA 2252571 A1 19971106; EP 1015116 A1 20000705
DOCDB simple family (application)
CA 9700302 W 19970501; AU 2378097 A 19970501; CA 2252571 A 19970501; EP 97919230 A 19970501