EP 1015558 A1 20000705 - METHODS AND DEVICES FOR THE LONG-TERM CULTURE OF HEMATOPOIETIC PROGENITOR CELLS
Title (en)
METHODS AND DEVICES FOR THE LONG-TERM CULTURE OF HEMATOPOIETIC PROGENITOR CELLS
Title (de)
VERFAHREN UND VORRICHTUNGEN ZUR LANGZEITKULTUR DER HÄMATOPOIETISCHEN VORLÄUFERZELLEN
Title (fr)
PROCEDES ET DISPOSITIFS DE CULTURE A LONG TERME DE CELLULES SOUCHES HEMATOPOIETIQUES
Publication
Application
Priority
- US 9820123 W 19980925
- US 5995497 P 19970925
Abstract (en)
[origin: EP1627914A2] The invention pertains to methods and devices for the long term, in vitro culture of hematopoietic progenitor cells in the absence of exogenously added hematopoietic growth factors, improved methods for the introduction of foreign genetic material into cells of hematopoietic origin, and to apparatus for performing these methods. The hematopoietic progenitor cells are cultured on a three-dimensional porous biomaterial. The three-dimensional porous biomaterial enhances hematopoietic progenitor cell survival and leads to an expansion of progenitor cell numbers and/or functionality, while maintaining progenitor cell pluripotency in the absence of exogenous growth factors. In addition, the three-dimensional porous biomaterial supports high level transduction on cells cultured upon such environment.
IPC 1-7
IPC 8 full level
C12M 3/00 (2006.01); C12M 3/04 (2006.01); C12N 5/06 (2006.01); C12N 5/0789 (2010.01); C12N 5/10 (2006.01)
CPC (source: EP)
C12M 23/20 (2013.01); C12M 25/14 (2013.01); C12M 29/10 (2013.01); C12N 5/0647 (2013.01); C12N 2501/125 (2013.01); C12N 2501/23 (2013.01); C12N 2501/26 (2013.01); C12N 2502/1394 (2013.01); C12N 2533/30 (2013.01); C12N 2533/52 (2013.01)
Citation (search report)
See references of WO 9915629A1
Designated contracting state (EPC)
BE CH DE DK ES FI FR GB IT LI NL SE
DOCDB simple family (publication)
EP 1627914 A2 20060222; EP 1627914 A3 20060301; AU 9582398 A 19990412; CA 2304650 A1 19990401; CN 1272878 A 20001108; EP 1015558 A1 20000705; JP 2001517428 A 20011009; WO 9915629 A1 19990401
DOCDB simple family (application)
EP 05108873 A 19980925; AU 9582398 A 19980925; CA 2304650 A 19980925; CN 98809535 A 19980925; EP 98949516 A 19980925; JP 2000512923 A 19980925; US 9820123 W 19980925