Global Patent Index - EP 1015558 A1

EP 1015558 A1 2000-07-05 - METHODS AND DEVICES FOR THE LONG-TERM CULTURE OF HEMATOPOIETIC PROGENITOR CELLS

Title (en)

METHODS AND DEVICES FOR THE LONG-TERM CULTURE OF HEMATOPOIETIC PROGENITOR CELLS

Title (de)

VERFAHREN UND VORRICHTUNGEN ZUR LANGZEITKULTUR DER HÄMATOPOIETISCHEN VORLÄUFERZELLEN

Title (fr)

PROCEDES ET DISPOSITIFS DE CULTURE A LONG TERME DE CELLULES SOUCHES HEMATOPOIETIQUES

Publication

EP 1015558 A1 (EN)

Application

EP 98949516 A

Priority

  • US 9820123 W
  • US 5995497 P

Abstract (en)

[origin: WO9915629A1] The invention pertains to methods and devices for the long term, in vitro culture of hematopoietic progenitor cells in the absence of exogenously added hematopoietic growth factors, improved methods for the introduction of foreign genetic material into cells of hematopoietic origin, and to apparatus for performing these methods. The hematopoietic progenitor cells are cultured on a three-dimensional porous biomaterial. The three-dimensional porous biomaterial enhances hematopoietic progenitor cell survival and leads to an expansion of progenitor cell numbers and/or functionality, while maintaining progenitor cell pluripotency in the absence of exogenous growth factors. In addition, the three-dimensional porous biomaterial supports high level transduction on cells cultured upon such environment.

IPC 1-7 (main, further and additional classification)

C12N 5/06; C12M 3/00

IPC 8 full level (invention and additional information)

C12M 3/00 (2006.01); C12M 3/04 (2006.01); C12N 5/0789 (2010.01); C12N 5/10 (2006.01)

CPC (invention and additional information)

C12M 25/14 (2013.01); C12M 23/20 (2013.01); C12M 29/10 (2013.01); C12N 5/0647 (2013.01); C12N 2501/125 (2013.01); C12N 2501/23 (2013.01); C12N 2501/26 (2013.01); C12N 2502/1394 (2013.01); C12N 2533/30 (2013.01); C12N 2533/52 (2013.01)

Citation (search report)

See references of WO 9915629A1

Designated contracting state (EPC)

BE CH DE DK ES FI FR GB IT LI NL SE

EPO simple patent family

WO 9915629 A1 19990401; AU 9582398 A 19990412; CA 2304650 A1 19990401; CN 1272878 A 20001108; EP 1015558 A1 20000705; JP 2001517428 A 20011009

INPADOC legal status


2006-05-17 [18R] REFUSED

- Effective date: 20050927

2003-11-05 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20030918

2000-07-05 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000425

2000-07-05 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): BE CH DE DK ES FI FR GB IT LI NL SE