EP 1015626 A1 20000705 - METHOD OF MONITORING THE COMPETITIVENESS OF A MICROBIAL STRAIN
Title (en)
METHOD OF MONITORING THE COMPETITIVENESS OF A MICROBIAL STRAIN
Title (de)
VERFAHREN ZUR ÜBERWACHUNG DER WETTBEWERBSFÄHIGKEIT EINES MIKROBIELLEN STAMMS
Title (fr)
PROCEDE DE CONTROLE DE LA COMPETITIVITE D'UNE SOUCHE MICROBIENNE
Publication
Application
Priority
- IB 9801411 W 19980911
- NO 974299 A 19970918
Abstract (en)
[origin: WO9914359A1] A method of selectively monitoring development and/or competitiveness of a microbial strain in a sample environment, comprising inserting a selectively detectable genetic label into the strain and adding the labelled strain to the sample environment and isolating DNA or RNA of said strain from the sample environment and detecting the amount of the genetic label. The method is also used for selecting, among a multiplicity of microbial strains, the strain that in a pre-selected environment and under pre-selected conditions has the highest capability to survive, multiply and/or express a pre-selected gene. The amount of genetic label is detected by PCR including a PCR technique that is capable of detecting a label consisting of a single nucleotide substitution, including a technique where the first PCR cycle involves the use of a reverse transcriptase. The method is also useful for establishing the identity and origin of a microbial strain by inserting a genetic label comprising a coded message.
IPC 1-7
C12Q 1/04; C12Q 1/68; C12N 1/00; C12N 1/20; C12N 1/21; A23C 9/127; A23C 19/032
IPC 8 full level
A23C 19/032 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6809 (2018.01); C12Q 1/6823 (2018.01); C12Q 1/6888 (2018.01)
CPC (source: EP)
A23C 19/0321 (2013.01); A23C 19/0323 (2013.01); C12N 15/10 (2013.01); C12Q 1/6809 (2013.01); C12Q 1/6823 (2013.01); C12Q 1/6888 (2013.01); A23C 2220/202 (2013.01); C12Q 2600/158 (2013.01)
Citation (search report)
See references of WO 9914359A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9914359 A1 19990325; AU 8880998 A 19990405; CA 2334007 A1 19990325; EP 1015626 A1 20000705; NO 974299 D0 19970918
DOCDB simple family (application)
IB 9801411 W 19980911; AU 8880998 A 19980911; CA 2334007 A 19980911; EP 98940492 A 19980911; NO 974299 A 19970918