Global patent index - EP 1017795 A1

EP 1017795 A1 2000-07-12 - DETECTING COW, SHEEP AND PIG PRIONS IN A SAMPLE AND TRANSGENIC ANIMAL USED FOR SAME

Title (en)

DETECTING COW, SHEEP AND PIG PRIONS IN A SAMPLE AND TRANSGENIC ANIMAL USED FOR SAME

Title (de)

DETEKTION VON RINDER-, SCHAF- UND SCHWEINE-PRIONEN IN EINER PROBE UND TRANSGENES TIER, WELCHES DAFÜR GEBRAUCHT WIRD

Title (fr)

DETECTION DES PRIONS DE VACHE, DE MOUTON, ET DE COCHON DANS UN ECHANTILLON, ET ANIMAUX TRANSGENIQUES UTILISES A CETTE FIN

Publication

EP 1017795 A1 (EN)

Application

EP 98945791 A

Priority

  • US 9817879 W
  • US 93536397 A

Abstract (en)

[origin: WO9915640A1] Transgenic animals are produced which animals have (1) their endogenous PrP gene ablated; and (2) have an exogenous PrP gene from a genetically diverse animal. The transgenic animal is preferably a mouse, rat or hamster with mice being particularly preferred. The exogenous PrP gene is preferably from a sheep, cow, or pig with cow PrP genes being particularly preferred. When a mouse of the invention is inoculated with a sample containing prions which generally only infects a genetically diverse species (e.g. a cow) the mouse will become ill within about 250 days or less. Methods of producing the transgenic animals are disclosed including (1) microinjecting a mouse egg (having an ablated endogenous PrP gene) with a bovine PrP gene, or (2) breeding a mouse with an ablated PrP gene with a mouse with a bovine PrP gene. Mice produced are used to test samples for the presence of prions which generally only infect cows.

IPC 1-7 (main, further and additional classification)

C12N 15/00

IPC 8 full level (invention and additional information)

A01K 67/027 (2006.01); C07K 14/47 (2006.01); C12N 15/09 (2006.01); C12N 15/85 (2006.01); G01N 33/15 (2006.01); G01N 33/50 (2006.01)

CPC (invention and additional information)

C12N 15/8509 (2013.01); A01K 67/0275 (2013.01); C07K 14/47 (2013.01); A01K 2217/05 (2013.01); A01K 2217/075 (2013.01); A01K 2227/105 (2013.01); A01K 2267/0318 (2013.01); A01K 2267/0337 (2013.01); A01K 2267/0343 (2013.01); G01N 2800/2828 (2013.01)

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE

EPO simple patent family

WO 9915640 A1 19990401; AU 752933 B2 20021003; AU 9295398 A 19990412; CA 2302836 A1 19990401; EP 1017795 A1 20000712; EP 1017795 A4 20020508; JP 2001517775 A 20011009

INPADOC legal status

2004-06-09 [18D] DEEMED TO BE WITHDRAWN

- Ref Legal Event Code: 18D

- Effective date: 20031202

2003-09-10 [17Q] FIRST EXAMINATION REPORT

- Ref Legal Event Code: 17Q

- Effective date: 20030722

2002-05-08 [A4] SUPPLEMENTARY SEARCH REPORT

- Ref Legal Event Code: A4

- Effective date: 20020325

2002-05-08 [AK] DESIGNATED CONTRACTING STATES:

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- Designated State(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE

2000-07-12 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 20000331

2000-07-12 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE