EP 1017825 A1 20000712 - METHODS OF IN SITU MODIFICATION OF PLANT GENES
Title (en)
METHODS OF IN SITU MODIFICATION OF PLANT GENES
Title (de)
METHODEN ZUR IN SITU-VERÄNDERUNG VON PFLANZENGENEN
Title (fr)
PROCEDES DE MODIFICATION IN SITU DE PHYTOGENES
Publication
Application
Priority
- GB 9801499 W 19980522
- GB 9711015 A 19970528
Abstract (en)
[origin: GB2326163A] A method of producing plants which exhibit an agronomically desirable trait comprises mutating or otherwise modifying in situ in a plant cell at least one gene and regenerating from a cell exhibiting the said trait fertile morphologically normal whole plants, and is characterised in that a polynucleotide is introduced into the plant cell, the said polynucleotide comprising at least one region which is substantially complementary to the gene, which gene when mutated or otherwise modified provides for the agronomically desirable trait, the region in the said polynucleotide containing at least one base mismatch in comparison with the like region in the said gene, so that the region in the said gene is altered by the DNA repair/replication system of the cell to include the said mismatch. The agronomically desirable trait may be herbicide resistance and specific mutations to the EPSPS and PDS genes are disclosed. The polynucleotide may contain RNA and DNA sequences and may have areas of heteroduplex, homoduplex and unpaired bases.
IPC 1-7
IPC 8 full level
A01H 1/00 (2006.01); C12N 5/10 (2006.01); C12N 9/10 (2006.01); C12N 15/09 (2006.01); C12N 15/54 (2006.01); C12N 15/82 (2006.01); C12N 15/90 (2006.01)
CPC (source: EP)
C12N 9/1092 (2013.01); C12N 15/8213 (2013.01); C12N 15/8274 (2013.01); C12N 15/8275 (2013.01); C12N 15/8278 (2013.01); C12N 15/902 (2013.01)
Citation (search report)
See references of WO 9854330A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
GB 2326163 A 19981216; GB 9811138 D0 19980722; AU 7541498 A 19981230; EP 1017825 A1 20000712; GB 9711015 D0 19970723; JP 2002503101 A 20020129; WO 9854330 A1 19981203
DOCDB simple family (application)
GB 9811138 A 19980522; AU 7541498 A 19980522; EP 98922954 A 19980522; GB 9711015 A 19970528; GB 9801499 W 19980522; JP 50036099 A 19980522