Global patent index - EP 1017841 A1

EP 1017841 A1 2000-07-12 - CHROMATOGRAPHIC METHOD FOR MUTATION DETECTION USING MUTATION SITE SPECIFICALLY ACTING ENZYMES

Title (en)

CHROMATOGRAPHIC METHOD FOR MUTATION DETECTION USING MUTATION SITE SPECIFICALLY ACTING ENZYMES

Title (de)

CHROMATOGRAPHISCHES VERFAHREN ZUR MUTATIONS-DETEKTION UNTER VERWENDUNG VON ENZYMEN DIE SPEZIFISCH AM MUTATIONSORT WIRKEN

Title (fr)

PROCEDE CHROMATOGRAPHIQUE DE DETECTION DE MUTATION PAR UTILISATION D'ENZYMES AGISSANT SPECIFIQUEMENT SUR LE SITE DE MUTATION

Publication

EP 1017841 A1 (EN)

Application

EP 98942090 A

Priority

  • US 9817062 W
  • US 5567697 P
  • US 6241397 P

Abstract (en)

[origin: WO9909203A1] A method for analyzing a sample of double stranded DNA to determine the presence of a mutation therein comprises contacting the sample with a mutation site binding reagent, and chromatographically separating and detecting the product. The chromatographic separation can be performed using Matched Ion Polynucleotide Chromatography, size exclusion chromatography, ion exchange chromatography, or reverse phase chromatography. The mutation site binding reagent can be an enzyme or a non-proteinaceous chemical reagent. In one embodiment, a mutation site binding reagent binds to the site of mutation and alters the chromatographic retention time. In another embodiment, a mutation site binding reagent cleaves at the site of mutation, resulting in an increase in the number of fragments.

IPC 1-7 (main, further and additional classification)

C12Q 1/00; C07H 19/04; C07H 21/00; C12P 19/34; C12Q 1/68

IPC 8 full level (invention and additional information)

C07H 19/04 (2006.01); C07H 21/00 (2006.01); C12N 15/09 (2006.01); C12P 19/34 (2006.01); C12Q 1/68 (2006.01); G01N 30/88 (2006.01); G01N 33/53 (2006.01); G01N 33/538 (2006.01); G01N 33/566 (2006.01)

CPC (invention and additional information)

C07H 21/00 (2013.01); C12Q 1/6827 (2013.01); G01N 33/5308 (2013.01); G01N 33/538 (2013.01)

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9909203 A1 19990225; AU 744476 B2 20020228; AU 9021998 A 19990308; CA 2298630 A1 19990225; EP 1017841 A1 20000712; EP 1017841 A4 20020109; JP 2001515217 A 20010918; JP 3345832 B2 20021118

INPADOC legal status

2005-06-22 [18D] DEEMED TO BE WITHDRAWN

- Ref Legal Event Code: 18D

- Effective date: 20041218

2004-07-21 [17Q] FIRST EXAMINATION REPORT

- Ref Legal Event Code: 17Q

- Effective date: 20040607

2002-01-09 [A4] SUPPLEMENTARY SEARCH REPORT

- Ref Legal Event Code: A4

- Effective date: 20011128

2002-01-09 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2000-07-12 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 20000310

2000-07-12 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE