Global Patent Index - EP 1017841 A1

EP 1017841 A1 20000712 - CHROMATOGRAPHIC METHOD FOR MUTATION DETECTION USING MUTATION SITE SPECIFICALLY ACTING ENZYMES

Title (en)

CHROMATOGRAPHIC METHOD FOR MUTATION DETECTION USING MUTATION SITE SPECIFICALLY ACTING ENZYMES

Title (de)

CHROMATOGRAPHISCHES VERFAHREN ZUR MUTATIONS-DETEKTION UNTER VERWENDUNG VON ENZYMEN DIE SPEZIFISCH AM MUTATIONSORT WIRKEN

Title (fr)

PROCEDE CHROMATOGRAPHIQUE DE DETECTION DE MUTATION PAR UTILISATION D'ENZYMES AGISSANT SPECIFIQUEMENT SUR LE SITE DE MUTATION

Publication

EP 1017841 A1 20000712 (EN)

Application

EP 98942090 A 19980818

Priority

  • US 9817062 W 19980818
  • US 5567697 P 19970818
  • US 6241397 P 19971014

Abstract (en)

[origin: WO9909203A1] A method for analyzing a sample of double stranded DNA to determine the presence of a mutation therein comprises contacting the sample with a mutation site binding reagent, and chromatographically separating and detecting the product. The chromatographic separation can be performed using Matched Ion Polynucleotide Chromatography, size exclusion chromatography, ion exchange chromatography, or reverse phase chromatography. The mutation site binding reagent can be an enzyme or a non-proteinaceous chemical reagent. In one embodiment, a mutation site binding reagent binds to the site of mutation and alters the chromatographic retention time. In another embodiment, a mutation site binding reagent cleaves at the site of mutation, resulting in an increase in the number of fragments.

IPC 1-7

C12Q 1/00; C12Q 1/68; C12P 19/34; C07H 21/00; C07H 19/04

IPC 8 full level

C07H 19/04 (2006.01); C07H 21/00 (2006.01); C12N 15/09 (2006.01); C12P 19/34 (2006.01); C12Q 1/68 (2006.01); G01N 30/88 (2006.01); G01N 33/53 (2006.01); G01N 33/538 (2006.01); G01N 33/566 (2006.01)

CPC (source: EP)

C07H 21/00 (2013.01); C12Q 1/6827 (2013.01); G01N 33/5308 (2013.01); G01N 33/538 (2013.01)

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 9909203 A1 19990225; AU 744476 B2 20020228; AU 9021998 A 19990308; CA 2298630 A1 19990225; EP 1017841 A1 20000712; EP 1017841 A4 20020109; JP 2001515217 A 20010918; JP 3345832 B2 20021118

DOCDB simple family (application)

US 9817062 W 19980818; AU 9021998 A 19980818; CA 2298630 A 19980818; EP 98942090 A 19980818; JP 2000509864 A 19980818