EP 1017852 A1 20000712 - OBTAINING NUCLEIC ACID SEQUENCES
Title (en)
OBTAINING NUCLEIC ACID SEQUENCES
Title (de)
VERFAHREN ZUR ISOLIERUNG VON NUKLEINSÄUREN
Title (fr)
OBTENTION DE SEQUENCES D'ACIDE NUCLEIQUE
Publication
Application
Priority
- GB 9802958 W 19981005
- GB 9720992 A 19971003
Abstract (en)
[origin: WO9918236A1] A method of obtaining target nucleic sequences derived from genes differentially expressed in a test cell sample as compared to a reference cell sample by the use of subtractive hybridisation. The duplex molecules formed during the subtractive hybridisation step are subjected to selective cross-linking and the unsubtracted, single stranded cDNA is used as a template to generate nucleic acid strands complementary thereto incorporating a binding ligand. These complementary strands are then immobilised on a solid phase support system having a specific binding partner for the ligand. The solid phase support system is then treated to remove non-bound nucleic acid and leave a pure sample of the bound complementary strand. This bound strand may be used as, or to generate, the target nucleic acid molecules.
IPC 1-7
IPC 8 full level
C12Q 1/68 (2006.01); C12Q 1/6809 (2018.01)
CPC (source: EP)
C12Q 1/6809 (2013.01)
Citation (search report)
See references of WO 9918236A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9918236 A1 19990415; AU 9449098 A 19990427; EP 1017852 A1 20000712; GB 9720992 D0 19971203
DOCDB simple family (application)
GB 9802958 W 19981005; AU 9449098 A 19981005; EP 98947647 A 19981005; GB 9720992 A 19971003