Global Patent Index - EP 1017852 A1

EP 1017852 A1 2000-07-12 - OBTAINING NUCLEIC ACID SEQUENCES

Title (en)

OBTAINING NUCLEIC ACID SEQUENCES

Title (de)

VERFAHREN ZUR ISOLIERUNG VON NUKLEINSÄUREN

Title (fr)

OBTENTION DE SEQUENCES D'ACIDE NUCLEIQUE

Publication

EP 1017852 A1 (EN)

Application

EP 98947647 A

Priority

  • GB 9802958 W
  • GB 9720992 A

Abstract (en)

[origin: WO9918236A1] A method of obtaining target nucleic sequences derived from genes differentially expressed in a test cell sample as compared to a reference cell sample by the use of subtractive hybridisation. The duplex molecules formed during the subtractive hybridisation step are subjected to selective cross-linking and the unsubtracted, single stranded cDNA is used as a template to generate nucleic acid strands complementary thereto incorporating a binding ligand. These complementary strands are then immobilised on a solid phase support system having a specific binding partner for the ligand. The solid phase support system is then treated to remove non-bound nucleic acid and leave a pure sample of the bound complementary strand. This bound strand may be used as, or to generate, the target nucleic acid molecules.

IPC 1-7 (main, further and additional classification)

C12Q 1/68

IPC 8 full level (invention and additional information)

C12Q 1/68 (2006.01)

CPC (invention and additional information)

C12Q 1/6809 (2013.01)

Citation (search report)

See references of WO 9918236A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9918236 A1 19990415; AU 9449098 A 19990427; EP 1017852 A1 20000712; GB 9720992 D0 19971203

INPADOC legal status


2001-06-27 [18W] WITHDRAWN

- Date of withdrawal: 20010419

2000-07-12 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000428

2000-07-12 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE