EP 1017856 A1 20000712 - METHOD AND KIT FOR EVALUATION OF HIV MUTATIONS
Title (en)
METHOD AND KIT FOR EVALUATION OF HIV MUTATIONS
Title (de)
VERFAHREN UND REAGENTIENSATZ ZUR ERMITTLUNG VON HIV MUTATIONEN
Title (fr)
PROCEDE ET ENSEMBLE DE MATERIEL D'EVALUATION DE MUTATIONS VIH
Publication
Application
Priority
- CA 9800913 W 19980928
- US 93864197 A 19970926
Abstract (en)
[origin: WO9916910A1] A streamlined, hierarchical method for obtaining information about the allelic type of a sample of genetic material derived from an HIV-infected sample relies on the observation that 93-95 % of the known mutational variants of the reverse transcriptase and protease genes of HIV-1 can be determined by evaluating the positions of the A and T nucleotides within the sample. Thus, a substantial fraction of all mutational variations can be unequivocally identified by performing two initial sequencing reactions on the sample in which only ddA and ddT are used as chain terminators. For the small fraction of samples which are not identifiable based on the positions of these two bases, a second test is performed in which the sequence is determined in the 3'-direction for all four bases. This test will identify substantially all of the remaining samples. For those for which an ambiguity remains, however, a final test in which the sequence of the sample is determined in both the 3' and 5-direction for all four bases in performed. To perform the method, reagents suitable for performing the three tests within the hierarchy are suitably packaged as a kit containing two or more sub-kits. The first sub-kit contains reagents for performing A and T sequencing. The additional sub-kits contain reagents for performing a four-base sequence determination on one or both strands of the target DNA.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/70 (2006.01)
CPC (source: EP)
C12Q 1/6818 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/703 (2013.01)
Citation (search report)
See references of WO 9916910A1
Designated contracting state (EPC)
DE ES FR GB SE
DOCDB simple family (publication)
WO 9916910 A1 19990408; AU 751471 B2 20020815; AU 9249398 A 19990423; CA 2302201 A1 19990408; EP 1017856 A1 20000712; JP 2001518313 A 20011016
DOCDB simple family (application)
CA 9800913 W 19980928; AU 9249398 A 19980928; CA 2302201 A 19980928; EP 98944941 A 19980928; JP 2000513978 A 19980928