EP 1018003 A4 20020417 - MODULATORS OF DNA CYTOSINE-5 METHYLTRANSFERASE AND METHODS FOR USE THEREOF
Title (en)
MODULATORS OF DNA CYTOSINE-5 METHYLTRANSFERASE AND METHODS FOR USE THEREOF
Title (de)
MODULATOREN DER CYTOSIN-5-METHYL-TRANSFERASE UND VERFAHREN ZU IHRER VERWENDUNG
Title (fr)
MODULATEURS DE CYTOSINE-5 METHYLTRANSFERASE ET PROCEDES DE LEUR UTILISATION
Publication
Application
Priority
- US 9812351 W 19980612
- US 5741197 P 19970829
Abstract (en)
[origin: WO9912027A1] A synthetic oligonucleotide comprising a C-5 methylcytosine and which recognizes and binds an allosteric site on DNA methyltransferase thereby inhibiting DNA methyltransferase activity is disclosed. Also disclosed is a composition comprising a synthetic oligonucleotide of the invention. The composition is useful for inhibiting DNA methyltransferase activity, thereby inhibiting the methylation of DNA. The composition can be a pharmaceutical composition useful for treating disorders associated with methylation defects, such as cancer and certain developmental disorders. Also disclosed is a method of inhibiting methylation of DNA. The method involves contacting a DCMTase with a synthetic oligonucleotide of the invention in the presence of the DNA, thereby resulting in an enzyme/synthetic oligonucleotide complex. The presence of the complex prevents catalysis, thereby inhibiting DNA methyltransferase activity. Also disclosed is a method of treating a disorder of cell proliferation or development by administering to a subject a synthetic oligonucleotide of the invention. The inhibition of DNA methyltransferase prevents the methylation of DNA thereby treating the disorder of cell proliferation or development.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12N 9/10 (2006.01); C12N 15/113 (2010.01); C12Q 1/48 (2006.01); A61K 38/00 (2006.01)
CPC (source: EP)
C12N 15/1137 (2013.01); A61K 38/00 (2013.01); C12N 2310/13 (2013.01); C12N 2310/334 (2013.01)
Citation (search report)
- [XY] EP 0756008 A2 19970129 - HEALTH RESEARCH INC [US], et al
- [A] US 5578716 A 19961126 - SZYF MOSHE [CA], et al
- [PX] WO 9744346 A2 19971127 - UNIV MCGILL [CA], et al
- [XY] FLYNN JAMES ET AL: "Murine DNA cytosine-C-5 methyltransferase: Pre-steady- and steady-state kinetic analysis with regulatory DNA sequences.", BIOCHEMISTRY, vol. 35, no. 23, 1996, pages 7308 - 7315, XP002189815, ISSN: 0006-2960
- [X] TOLLEFSBOL TRYGVE O ET AL: "Control of methylation spreading in synthetic DNA sequences by the murine DNA methyltransferase.", JOURNAL OF MOLECULAR BIOLOGY, vol. 269, no. 4, 20 June 1997 (1997-06-20), pages 494 - 504, XP002189816, ISSN: 0022-2836
- [PX] FLYNN JAMES ET AL: "DNA binding discrimination of the murine DNA cytosine-C5 methyltransferase.", JOURNAL OF MOLECULAR BIOLOGY, vol. 279, no. 1, 29 May 1998 (1998-05-29), pages 101 - 116, XP002189817, ISSN: 0022-2836
- See references of WO 9912027A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9912027 A1 19990311; AT E294388 T1 20050515; CA 2300354 A1 19990311; DE 69829984 D1 20050602; EP 1018003 A1 20000712; EP 1018003 A4 20020417; EP 1018003 B1 20050427; JP 2001514862 A 20010918
DOCDB simple family (application)
US 9812351 W 19980612; AT 98930207 T 19980612; CA 2300354 A 19980612; DE 69829984 T 19980612; EP 98930207 A 19980612; JP 2000508978 A 19980612