EP 1019711 A4 20010613 - METHODS AND MATERIALS FOR OPTIMIZATION OF ELECTRONIC HYBRIDIZATION REACTIONS
Title (en)
METHODS AND MATERIALS FOR OPTIMIZATION OF ELECTRONIC HYBRIDIZATION REACTIONS
Title (de)
VERFAHREN UND MATERIALIEN ZUR OPTIMIERUNG DER ELEKTRONISCHEN HYBRIDISIERUNGSREAKTION
Title (fr)
PROCEDES ET MATERIELS POUR OPTIMISER DES REACTIONS D'HYBRIDATION ELECTRONIQUES
Publication
Application
Priority
- US 9714489 W 19970818
- US 70826296 A 19960906
Abstract (en)
[origin: WO9810273A1] The following inventions relate to discoveries concerning the various parameters, electrolytes (buffers), and other conditions which improve or optimize the speed of DNA transport, the efficiency of DNA hybridization reactions, and the overall hybridization specificity in microelectronic chips and devices. In particular, this invention relates to the discovery that low conductance zwitterionic buffer solutions, especially those containing the amino acid Histidine prepared at concentrations of SIMILAR 50 mM and at or near the pI (isoelectric point SIMILAR pH 7.47), provide optimal conditions for both rapid electrophoretic DNA transport and efficient hybridization reactions. Hybridization efficiencies of at least a factor of 10 relative to the next best known buffer, Cysteine, are achieved. Test data demonstrate an approximately 50,000 fold increase in hybridization efficiency compared to Cysteine.
IPC 1-7
IPC 8 full level
C12M 1/00 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); G01N 27/447 (2006.01); G01N 33/53 (2006.01); G01N 33/566 (2006.01)
CPC (source: EP KR)
C12Q 1/68 (2013.01 - EP); C12Q 1/6832 (2013.01 - EP); G01N 27/26 (2013.01 - KR); H01L 29/6656 (2013.01 - EP)
Citation (search report)
- [Y] WO 9601836 A1 19960125 - NANOGEN INC [US]
- [Y] WO 9615576 A1 19960523 - SARNOFF DAVID RES CENTER [US]
- [T] EDMAN C ET AL: "Electric field directed nucleic acid hybridization on microchips", NUCLEIC ACIDS RESEARCH, vol. 25, no. 24, 1997, pages 4907 - 14, XP002165640
- See references of WO 9810273A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9810273 A1 19980312; AU 4071997 A 19980326; AU 723564 B2 20000831; BR 9712800 A 19991123; CA 2264780 A1 19980312; CA 2264780 C 20060801; CN 1180248 C 20041215; CN 1230255 A 19990929; EP 1019711 A1 20000719; EP 1019711 A4 20010613; JP 2001501301 A 20010130; JP 4213216 B2 20090121; KR 100591626 B1 20060620; KR 20010029477 A 20010406; NZ 334314 A 20000929
DOCDB simple family (application)
US 9714489 W 19970818; AU 4071997 A 19970818; BR 9712800 A 19970818; CA 2264780 A 19970818; CN 97197960 A 19970818; EP 97938378 A 19970818; JP 51267698 A 19970818; KR 19997001868 A 19990305; NZ 33431497 A 19970818