Global Patent Index - EP 1021462 A1

EP 1021462 A1 2000-07-26 - ASSAYS FOR NUCLEAR RECEPTOR LIGANDS USING FRET

Title (en)

ASSAYS FOR NUCLEAR RECEPTOR LIGANDS USING FRET

Title (de)

TEST FÜR KERNREZEPTOR-LIGANDEN UNTER VERWENDUNG VON FRET

Title (fr)

ESSAIS POUR LIGANDS A RECEPTEURS NUCLEAIRES REPOSANT SUR LE TRANSFERT D'ENERGIE DE RESONANCE EN FLUORESCENCE

Publication

EP 1021462 A1 (EN)

Application

EP 98956094 A

Priority

  • US 9821049 W
  • US 6138597 P

Abstract (en)

[origin: WO9918124A1] Provided is a method of identifying agonists and antagonists of nuclear receptors that comprises measuring agonist-dependent fluorescence resonance energy transfer (FRET) between a fluorescently labeled nuclear receptor or ligand binding domain and fluorescently labeled CREB-binding protein (CBP), p300, other nuclear co-activator, or binding portion thereof. The method is simple, rapid, and inexpensive. Nuclear receptors and nuclear receptor co-activators labeled with fluorescent reagents for use in the above-described method are also provided.

IPC 1-7 (main, further and additional classification)

C07K 14/435; C07K 14/705; C09K 11/06; G01N 33/53; G01N 33/566

IPC 8 full level (invention and additional information)

C07K 14/435 (2006.01); C07K 14/475 (2006.01); C07K 14/705 (2006.01); C09K 11/06 (2006.01); G01N 33/536 (2006.01); G01N 33/542 (2006.01); G01N 33/566 (2006.01); G01N 33/58 (2006.01); G01N 33/68 (2006.01)

CPC (invention and additional information)

C07K 14/475 (2013.01); C07K 14/705 (2013.01); G01N 33/542 (2013.01); G01N 33/566 (2013.01); G01N 33/6875 (2013.01); G01N 2333/70567 (2013.01); G01N 2500/00 (2013.01)

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE

EPO simple patent family

WO 9918124 A1 19990415; CA 2305711 A1 19990415; EP 1021462 A1 20000726; EP 1021462 A4 20050413; JP 2001519525 A 20011023

INPADOC legal status


2008-07-09 [18D] DEEMED TO BE WITHDRAWN

- Ref Legal Event Code: 18D

- Effective date: 20071204

2007-06-20 [17Q] FIRST EXAMINATION REPORT

- Ref Legal Event Code: 17Q

- Effective date: 20060915

2006-10-18 [17Q] FIRST EXAMINATION REPORT

- Ref Legal Event Code: 17Q

- Effective date: 20060915

2005-04-13 [A4] SUPPLEMENTARY SEARCH REPORT

- Ref Legal Event Code: A4

- Effective date: 20050225

2000-07-26 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 20000508

2000-07-26 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE