Global Patent Index - EP 1021462 A4

EP 1021462 A4 20050413 - ASSAYS FOR NUCLEAR RECEPTOR LIGANDS USING FRET

Title (en)

ASSAYS FOR NUCLEAR RECEPTOR LIGANDS USING FRET

Title (de)

TEST FÜR KERNREZEPTOR-LIGANDEN UNTER VERWENDUNG VON FRET

Title (fr)

ESSAIS POUR LIGANDS A RECEPTEURS NUCLEAIRES REPOSANT SUR LE TRANSFERT D'ENERGIE DE RESONANCE EN FLUORESCENCE

Publication

EP 1021462 A4 20050413 (EN)

Application

EP 98956094 A 19981006

Priority

  • US 9821049 W 19981006
  • US 6138597 P 19971007

Abstract (en)

[origin: WO9918124A1] Provided is a method of identifying agonists and antagonists of nuclear receptors that comprises measuring agonist-dependent fluorescence resonance energy transfer (FRET) between a fluorescently labeled nuclear receptor or ligand binding domain and fluorescently labeled CREB-binding protein (CBP), p300, other nuclear co-activator, or binding portion thereof. The method is simple, rapid, and inexpensive. Nuclear receptors and nuclear receptor co-activators labeled with fluorescent reagents for use in the above-described method are also provided.

IPC 1-7

C07K 14/435; C07K 14/705; C09K 11/06; G01N 33/53; G01N 33/566

IPC 8 full level

C07K 14/435 (2006.01); C07K 14/475 (2006.01); C07K 14/705 (2006.01); C09K 11/06 (2006.01); G01N 33/536 (2006.01); G01N 33/542 (2006.01); G01N 33/566 (2006.01); G01N 33/58 (2006.01); G01N 33/68 (2006.01)

CPC (source: EP)

C07K 14/475 (2013.01); C07K 14/705 (2013.01); G01N 33/542 (2013.01); G01N 33/566 (2013.01); G01N 33/6875 (2013.01); G01N 2333/70567 (2013.01); G01N 2500/00 (2013.01)

Citation (search report)

  • [E] WO 9856806 A1 19981217 - UNIV CALIFORNIA [US], et al
  • [E] WO 9941608 A2 19990819 - PROLIFIX LTD [GB], et al
  • [A] MATHIS G: "PROBING MOLECULAR INTERACTIONS WITH HOMOGENEOUS TECHNIQUES BASED ONRARE EARTH CRYPTATES AND FLUORESCENCE ENERGY TRANSFER", CLINICAL CHEMISTRY, vol. 41, no. 9, 1995, pages 1391 - 1397, XP001018128, ISSN: 0009-9147
  • [A] DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 22 July 1997 (1997-07-22), BELLANGER L ET AL: "Homogeneous time-resolved fluoroimmunoassay for cardiac troponin-I using TRACE technology", XP008042758, Database accession no. PREV199799633726 & CLINICAL CHEMISTRY, vol. 43, no. 6 PART 2, 1997, 49TH ANNUAL MEETING OF THE AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY; ATLANTA, GEORGIA, USA; JULY 20-24, 1997, pages S159, ISSN: 0009-9147
  • [A] ROGERS M V: "LIGHT ON HIGH-THROUGHPUT SCREENING: FLUORESCENCE-BASED ASSAY TECHNOLOGIES", DRUG DISCOVERY TODAY, ELSEVIER SCIENCE LTD, GB, vol. 2, no. 4, April 1997 (1997-04-01), pages 156 - 160, XP002916138, ISSN: 1359-6446
  • [A] KOLB A J ET AL: "HOMOGENEOUS, TIME-RESOLVED FLUORESCENCE METHOD FOR DRUG DISCOVERY", 1997, HIGH-THROUGHPUT SCREENING, THE DISCOVERY OF BIOACTIVE SUBSTANCES, XX, XX, PAGE(S) 345-360, XP008042717
  • [A] KOLB J M ET AL: "USE OF A NOVEL HOMOGENEOUS FLUORESCENT TECHNOLOGY IN HIGH THROUGHPUT SCREENING", JOURNAL OF BIOMOLECULAR SCREENING, LARCHMONT, NY, US, vol. 1, no. 4, 1996, pages 203 - 210, XP008042702, ISSN: 1087-0571
  • [A] LEE S P ET AL: "CHARACTERIZATION OF ENDONUCLEOLYTIC ACTIVITY OF HIV-1 INTEGRASE USING A FLUOROGENIC SUBSTRATE", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 227, 1995, pages 295 - 301, XP002945389, ISSN: 0003-2697
  • [T] ZHOU G ET AL: "Use of homogeneous time-resolved fluorescence energy transfer in the measurement of nuclear receptor activation", METHODS : A COMPANION TO METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC., NEW YORK, NY, US, vol. 25, no. 1, September 2001 (2001-09-01), pages 54 - 61, XP002267175, ISSN: 1046-2023
  • See references of WO 9918124A1

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE

DOCDB simple family (publication)

WO 9918124 A1 19990415; CA 2305711 A1 19990415; EP 1021462 A1 20000726; EP 1021462 A4 20050413; JP 2001519525 A 20011023

DOCDB simple family (application)

US 9821049 W 19981006; CA 2305711 A 19981006; EP 98956094 A 19981006; JP 2000514931 A 19981006