Global Patent Index - EP 1021538 A1

EP 1021538 A1 2000-07-26 - YEAST CELLS EXPRESSING MODIFIED G PROTEINS AND METHODS OF USE THEREFOR

Title (en)

YEAST CELLS EXPRESSING MODIFIED G PROTEINS AND METHODS OF USE THEREFOR

Title (de)

MODIFIZIERTE G PROTEINE-EXPRIMIERENDE HEFEZELLEN UND IHRE VERWENDUNGEN

Title (fr)

CELLULES DE LEVURE EXPRIMANT DES PROTEINES MODIFIEES G ET PROCEDES D'UTILISATION ASSOCIES

Publication

EP 1021538 A1 (EN)

Application

EP 98952143 A

Priority

  • US 9821168 W
  • US 94629897 A

Abstract (en)

[origin: WO9918211A1] The present invention pertains to novel yeast cells which are useful for the expression of heterologous G protein coupled receptors. The yeast cells of the present invention can be used in screening assays which can be used to screen for modulators of G protein coupled receptors. Specifically, the invention provides novel yeast cells which express a heterologous G protein coupled receptor and mutant and/or chimeric G protein subunit molecules which serve to functionally integrate the heterologous into the pheromone signaling pathway of the yeast cell. The invention also provides for the expression of heterologous G protein coupled receptors which are functionally integrated into the yeast cell membrane using a yeast alpha factor leader sequence. Drug discovery assays using the subject yeast cells are also provided.

IPC 1-7 (main, further and additional classification)

C12N 15/12; C07K 14/395; C07K 14/47; C12N 1/19; C12N 15/31; C12N 15/62; C12Q 1/02; C12Q 1/68; G01N 33/50

IPC 8 full level (invention and additional information)

C07K 14/395 (2006.01); C07K 14/47 (2006.01); C12N 1/19 (2006.01); C12N 15/09 (2006.01); C12N 15/12 (2006.01); C12Q 1/02 (2006.01); C12Q 1/68 (2006.01); G01N 33/50 (2006.01)

CPC (invention and additional information)

C07K 14/4722 (2013.01); C07K 14/395 (2013.01); C07K 2319/00 (2013.01)

Citation (search report)

See references of WO 9918211A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9918211 A1 19990415; AU 9791198 A 19990427; CA 2305204 A1 19990415; EP 1021538 A1 20000726; JP 2001519157 A 20011023; JP 2009143932 A 20090702

INPADOC legal status


2010-04-21 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20091027

2007-05-02 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20040308

2004-04-21 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20040308

2000-07-26 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000508

2000-07-26 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2000-07-26 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO

- Free text: AL PAYMENT 20000508;LT PAYMENT 20000508;LV PAYMENT 20000508;MK PAYMENT 20000508;RO PAYMENT 20000508;SI PAYMENT 20000508