Global Patent Index - EP 1021543 A1

EP 1021543 A1 2000-07-26 - MUTANTS OF YEAST CDC24P, DEFECTIVE IN BINDING OF THE G-PROTEIN BETA SUBUNIT

Title (en)

MUTANTS OF YEAST CDC24P, DEFECTIVE IN BINDING OF THE G-PROTEIN BETA SUBUNIT

Title (de)

MUTANTEN DES HEFENPROTEINS CDC24P, WELCHE ZUR BINDUNG MIT DER G-PROTEIN BETA-UNTEREINHEIT UNFÄHIG SIND

Title (fr)

MUTANTS DE LEVURE Cdc2p, DEFICIENTS DANS LA LIAISON DE LA SOUS-UNITE BETA DE PROTEINE G

Publication

EP 1021543 A1 (EN)

Application

EP 98946598 A

Priority

  • GB 9803033 W
  • GB 9721357 A
  • GB 9721358 A
  • GB 9812793 A

Abstract (en)

[origin: GB2330142A] There is described a nucleic acid sequence encoding a section of Cdc24p (from Saccharomyces cerevisiae), a GEF, which does not substantially alter the interaction of this protein with the subunit of a hetero-trimeric G protein (G#). Mutant nucleic acid libraries and peptides of Cdc24p which alter the interaction of this GEF and G# are illustrated. The interruption of Cdc24p and G# prevents mating in yeasts but allows vegetative growth. The use of the products to screen for anti-fungal or anti-cancer agents is envisaged. Mutants of the G#, Ste4p, which prevent the interaction of G# and Cdc24p, are illustrated. The binding of the Far1 protein to Cdc24p is described. An assay is described in which the effect of human oncogenes proto-Dbl and Vav on S. cerevisiae with mating defects is assessed. A dbl peptide is also exemplified.

IPC 1-7 (main, further and additional classification)

C12N 15/31; A61K 38/16; C07K 14/82

IPC 8 full level (invention and additional information)

G01N 33/566 (2006.01); A61K 38/00 (2006.01); A61K 45/00 (2006.01); A61P 43/00 (2006.01); C07K 14/82 (2006.01); C12N 15/09 (2006.01); C12N 15/31 (2006.01); C12Q 1/68 (2006.01)

CPC (invention and additional information)

C07K 14/82 (2013.01); A61K 38/00 (2013.01)

Citation (search report)

See references of WO 9918213A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

GB 2330142 A 19990414; GB 2330142 B 20000223; GB 9821998 D0 19981202; AU 9359998 A 19990427; CA 2305707 A1 19990415; EP 1021543 A1 20000726; JP 2001519158 A 20011023; WO 9918213 A1 19990415; WO 9918213 A8 19990520

INPADOC legal status


2005-03-09 [18D] DEEMED TO BE WITHDRAWN

- Ref Legal Event Code: 18D

- Effective date: 20040727

2004-04-28 [17Q] FIRST EXAMINATION REPORT

- Ref Legal Event Code: 17Q

- Effective date: 20040315

2000-07-26 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 20000505

2000-07-26 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE