Global patent index - EP 1025113 A1

EP 1025113 A1 2000-08-09 - IMPROVED PROCESS FOR PERFORMING POLYNUCLEOTIDE SEPARATIONS

Title (en)

IMPROVED PROCESS FOR PERFORMING POLYNUCLEOTIDE SEPARATIONS

Title (de)

VERFAHREN ZUR DURCHFÜHRUNG VON POLYNUKLEOTID-TRENNUNGEN

Title (fr)

PROCEDE AMELIORE PERMETTANT D'EFFECTUER DES SEPARATIONS DE POLYNUCLEOTIDES

Publication

EP 1025113 A1 (EN)

Application

EP 98923524 A

Priority

  • US 9810228 W
  • US 4912397 P
  • US 6383597 P

Abstract (en)

[origin: WO9856798A1] The invention recognizes the deleterious effects of trace, and even undetectable amounts of multivalent cations on the separation of mixtures of polynucleotides, especially double stranded polynucleotides, and provides an improved method for separating such mixtures on wide pore, non-polar separation media by eliminating multivalent cations from the all aspects of the separation process. This is accomplished by using components in the separation process which are materials which do not release metal cations. In addition, the use of cation capture resins and other methods to remove residual traces of multivalent cations from eluting solvents, sample solutions, separation media, and system components is described. It is also important to remove any traces or organic contaminants from solvents solutions and system parts. Taking similar steps to remove residual traces of multivalent cations and organic impurities from the separation process, the invention may also be used in a batch process to separate mixtures of polynucleotides fragments.

IPC 1-7 (main, further and additional classification)

C07H 1/00

IPC 8 full level (invention and additional information)

C12N 15/00 (2006.01); B01D 15/08 (2006.01); C07H 1/00 (2006.01); C07H 1/06 (2006.01); C07H 21/00 (2006.01); C07H 21/04 (2006.01)

CPC (invention and additional information)

B01D 15/08 (2013.01); C07H 1/00 (2013.01); C07H 1/06 (2013.01); C07H 21/00 (2013.01); C07H 21/04 (2013.01); C12N 15/00 (2013.01)

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9856798 A1 19981217; AU 743789 B2 20020207; AU 7579698 A 19981230; CA 2288813 A1 19981217; EP 1025113 A1 20000809; EP 1025113 A4 20020109; JP 2001524133 A 20011127

INPADOC legal status

2004-06-09 [18D] DEEMED TO BE WITHDRAWN

- Ref Legal Event Code: 18D

- Effective date: 20031202

2002-02-27 [17Q] FIRST EXAMINATION REPORT

- Ref Legal Event Code: 17Q

- Effective date: 20020115

2002-01-09 [A4] SUPPLEMENTARY SEARCH REPORT

- Ref Legal Event Code: A4

- Effective date: 20011127

2002-01-09 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2002-01-09 [RIC1] CLASSIFICATION (CORRECTION)

- Ref Legal Event Code: RIC1

- Free Format Text: 7C 07H 1/00 A, 7B 01D 15/08 B

2000-08-09 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 19991231

2000-08-09 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE