EP 1025113 A4 20020109 - IMPROVED PROCESS FOR PERFORMING POLYNUCLEOTIDE SEPARATIONS
Title (en)
IMPROVED PROCESS FOR PERFORMING POLYNUCLEOTIDE SEPARATIONS
Title (de)
VERFAHREN ZUR DURCHFÜHRUNG VON POLYNUKLEOTID-TRENNUNGEN
Title (fr)
PROCEDE AMELIORE PERMETTANT D'EFFECTUER DES SEPARATIONS DE POLYNUCLEOTIDES
Publication
Application
Priority
- US 9810228 W 19980519
- US 4912397 P 19970610
- US 6383597 P 19971030
Abstract (en)
[origin: WO9856798A1] The invention recognizes the deleterious effects of trace, and even undetectable amounts of multivalent cations on the separation of mixtures of polynucleotides, especially double stranded polynucleotides, and provides an improved method for separating such mixtures on wide pore, non-polar separation media by eliminating multivalent cations from the all aspects of the separation process. This is accomplished by using components in the separation process which are materials which do not release metal cations. In addition, the use of cation capture resins and other methods to remove residual traces of multivalent cations from eluting solvents, sample solutions, separation media, and system components is described. It is also important to remove any traces or organic contaminants from solvents solutions and system parts. Taking similar steps to remove residual traces of multivalent cations and organic impurities from the separation process, the invention may also be used in a batch process to separate mixtures of polynucleotides fragments.
IPC 1-7
IPC 8 full level
C12N 15/00 (2006.01); B01D 15/08 (2006.01); C07H 1/00 (2006.01); C07H 1/06 (2006.01); C07H 21/00 (2006.01); C07H 21/04 (2006.01)
CPC (source: EP)
B01D 15/166 (2013.01); B01D 15/265 (2013.01); C07H 1/06 (2013.01); C07H 21/00 (2013.01); C07H 21/04 (2013.01); C12N 15/101 (2013.01)
Citation (search report)
- [A] US 5482628 A 19960109 - SCHICK HANS G [US]
- [A] US 5338448 A 19940816 - GJERDE DOUGLAS T [US]
- See references of WO 9856798A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9856798 A1 19981217; AU 743789 B2 20020207; AU 7579698 A 19981230; CA 2288813 A1 19981217; EP 1025113 A1 20000809; EP 1025113 A4 20020109; JP 2001524133 A 20011127
DOCDB simple family (application)
US 9810228 W 19980519; AU 7579698 A 19980519; CA 2288813 A 19980519; EP 98923524 A 19980519; JP 50248799 A 19980519